Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Vet. Pharmacology

This investigation was carried out to elucidate the influence of turmeric pre-treatment on the pharmacokinetic of marbofloxacin following single oral and intravenous administration in broiler chickens. Chickens were divided into four groups of seven each. Group-I and II were administered marbofloxacin (5 mg/kg body weight {b.w.}) intravenously and orally, while animals in group-III and IV received similar dose of marbofloxacin (5 mg/kg b.w.) intravenously and orally, after oral pre-treatment with turmeric (100 mg/kg b. w. per day, 10 days). Blood samples were collected from the right wing vein at 0 (blank sample), 0.166, 0.25, 0.33, 0.5, 0.75, 1, 2, 4, 6, 8, 10, 12, 18, 24 and 48 hr in all groups. The Serum concentrations of marbofloxacin were determined by a reverse phase high-performance liquid chromatography (HPLC) with UV detection at 295 nm. The serum concentrations were significantly higher in turmeric treated chickens following oral and intravenous routes. The pharmacokinetic data revealed that turmeric treated chickens had significantly higher area under curve (AUC), volume of distribution (Vc) and mean residential time (MRT). After oral dosing the absorption rate constant (kab) is significantly higher than the elimination rate constant (kel). This could result in the presence of in vivo flip-flop pharmacokinetics. The delayed absorption was evident following oral administration, which limited the elimination and demonstrated sustained release from entero-hepatic circulation Turmeric ameliorates the systemic and relative bioavailabilities of marbofloxacin after oral administrations in broiler chickens

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