Thesis (Ph.D.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Application in Medical and Biological

Background: Pathophysiological similarity exists between Gestational diabetes mellitus (GDM) and type 2 Diabetes Mellitus (T2DM) with common genetic origin. Genetic liability for GDM in our own population is still not researched. The goal of the study was to reveal the genotypic and allele frequency differences of 2 Single nucleotide polymorphisms (SNP)s namely, CDKAL1 (rs7754840) and CDKN2A/2B (rs10811661) between GDM pregnancies and normal pregnancies by Real time polymerase chain reaction (PCR) using Taqman allelic discrimination assays. Study Design: This study included 98 pregnant women in their second trimester of 24{u2013}28 gestational weeks and age group between 18 and 45 years. The diagnosis of GDM was based on the International Association of Diabetes and Pregnancy Study Groups criteria. According to the results of their oral glucose tolerance test, the women were divided into two groups: 47 pregnant women with GDM and 51pregnant women with normal glucose tolerance ((NGT)). Methods: DNA analysis for CDKAL1 (rs7754840) and CDKN2A/2B (rs10811661) Screening of rs7754840 and rs10811661 was performed with the TaqMan® allelic discrimination assay (TaqMan® universal Master Mix, no UNG, Part No.: 4440043, Applied Biosystems, Foster City, CA). The genotyping reaction was amplified on a GeneAmp PCR system 2700

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