Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Ornamental Horticulture

The present study was carried out at the Experimental Nursery of the Ornamental Horticulture Department, and Plant Tissue Culture Laboratory of Vegetable Crops Department, Faculty of Agriculture, Cairo University and , Cairo university, to study the micro propagation behavior and investigate the effect of helium neon laser radiation on stimulation of vegetative growth and anatomical structure of Moringa oleifera seeds by using different power levels (5, 10 and 15) with different time exposure (1, 3 and 5 min.). The results showed that the best survival rate of sterilized seeds was obtained when using 1.5% NaOCl with 30 min. soaking period. 1/2 MS-medium supplemented with 3 mM BA and1 mM Kin treatment produced the highest number of shoots.The greatest rooting percentage was recorded on medium supplemented with 2.5NAA mM without adding IBA.The combination treatment between 0.5 mM IBA and 2.5 mM NAA achieved the largest number of roots. Treating the moringa seeds by He-Ne laser to 5min. time exposure led to the best results in number of branches and leaves for both seasons.15mW at 3 min. gave the highest value of root length

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