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Evaluation of cell responses against hepatitis C virus antigens among Egyptian healthcare workers at high risk of infection /

Zainab Zakaria Ali Zenhom

Evaluation of cell responses against hepatitis C virus antigens among Egyptian healthcare workers at high risk of infection / تقييم رد الفعل الخلوى ضد أنتيجينات فيروس الإلتهاب الكبدى (سى) بين العاملين المصريين فى مجال الرعاية الصحية و المعرضين لمخاطر عالية من الإصابة بالعدوى Zainab Zakaria Ali Zenhom ; Supervised Mahmoud Ahmed Amer , Sayed Fekry Abdelwahab - Cairo : Zainab Zakaria Ali Zenhom , 2014 - 171 P. : charts , facsimiles ; 25cm

Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Zoology

Hepatitis C Virus (HCV) specific cell-mediated immunity (CMI) has been reported among exposed individuals without viremia or seroconversion. Limited data are available regarding CMI among at - risk, seronegative, aviremic Egyptian healthcare workers (HCW), where HCV genotype 4 predominates. We investigated CMI responses among HCW at the National liver institute, where over 85% of the patients are HCV - infected. We quantified HCV - specific CMI in 52 seronegative aviremic Egyptian HCW using an interferon gamma (IFN - Þ) enzyme-linked immunospot (ELISpot) assay in response to 7 HCV genotype 4a overlapping 15mer peptide pools covering most of the viral genome. We also characterized the phenotype of the responding T cells to the HCV peptide pools by flow cytometry



Cell mediated immunity HCV Healthcare workers