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Physiological studies to enhance the production of secondary metabolites using agrobacterium rhizogenes and in vitro hairy root formation on some medicinal plants /

Alia Ahmed Amer

Physiological studies to enhance the production of secondary metabolites using agrobacterium rhizogenes and in vitro hairy root formation on some medicinal plants / دراسات فسيولوجية لانتاج المركبات الثانوية الفعالة بزيادة النموات الجذرية معمليا بواسطة الاجروباكتيريا فى بعض النباتات الطبية Alia Ahmed Amer ; Supervised Mohamed Ramadan Abuelella Nesiem , Ayman Yehia Amin , Shadia Kotb Ahmed - Cairo : Alia Ahmed Amer , 2014 - 165 P. : chartes , facsimiles ; 25cm

Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agricultural Botany

This investigation was carried out during the period from 2010 until 2014 in Central Lab of Plant Physiology, Faculty Agriculture Cairo University Giza, Egypt. This work was conducted to find suitable protocol to increase the productivity of Hyoscyamus muticus active ingredients (hyoscyamine and scopolamine) through hairy roots technique using gene transformation with different strains of Agrobacterium rhizogenes. Optimization of callus culture conditions from leaf explants of H. muticus as well as comparing the alkaloids (hyoscyamine and scopolamine) concentrations from different sources, i.e. three clones of hairy root cultures, callus cultures and intact normal H. muticus plants



Agrobacterium rhizogenes Hairy root culture Hyoscyamus muticus