Biochemical and molecular studies on the immune system in association with a zoonotic bacterial infection in aquaculture /
Marwa Mohamed Mohamed Mohamed Abdalla
Biochemical and molecular studies on the immune system in association with a zoonotic bacterial infection in aquaculture / دراسات بيوكيميائية وجزيئية على النظام المناعى المصاحب للعدوى البكتيرية الزونوتيكية فى المزارع المائية Marwa Mohamed Mohamed Mohamed Abdalla ; Supervised Fathy Abdalgalel , Iman M. kamel Abumourad - Cairo : Marwa Mohamed Mohamed Mohamed Abdalla , 2014 - 184 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry
The present study presents the expression of A2M gene in the O. niloticus and albino rats as an immune response to their infection with Zoonotic bacteria. The infection established in fish with IP injection of 0.3 ml of 3X108 CFUml of C. perfringens, and in rats by feeding on insufficient cooked infected O. niloticus, Biochemical and molecular studies were established after 3- and 6- days post challenge. Liver functions (ALT, AST and ALP) were significantly (P0.05) highly elevated. Kidney functions (Urea and uric acid) were not affected but the Cr. showed significant increase in its level (P0.05). And the Ca. showed significant decrease in its level (P0.05). CRP elevated in rats and O. niloticus. Expression pattern of A2M gene was investigated using a semi- quantitative RT-PCR in liver, spleen and kidney tissues, which showed high expression after the bacterial infection indicating that A2M is immune responder against C. perfringens infection
Alpha 2-Macroglobulin Food- borne disease Zoonotic Disease
Biochemical and molecular studies on the immune system in association with a zoonotic bacterial infection in aquaculture / دراسات بيوكيميائية وجزيئية على النظام المناعى المصاحب للعدوى البكتيرية الزونوتيكية فى المزارع المائية Marwa Mohamed Mohamed Mohamed Abdalla ; Supervised Fathy Abdalgalel , Iman M. kamel Abumourad - Cairo : Marwa Mohamed Mohamed Mohamed Abdalla , 2014 - 184 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry
The present study presents the expression of A2M gene in the O. niloticus and albino rats as an immune response to their infection with Zoonotic bacteria. The infection established in fish with IP injection of 0.3 ml of 3X108 CFUml of C. perfringens, and in rats by feeding on insufficient cooked infected O. niloticus, Biochemical and molecular studies were established after 3- and 6- days post challenge. Liver functions (ALT, AST and ALP) were significantly (P0.05) highly elevated. Kidney functions (Urea and uric acid) were not affected but the Cr. showed significant increase in its level (P0.05). And the Ca. showed significant decrease in its level (P0.05). CRP elevated in rats and O. niloticus. Expression pattern of A2M gene was investigated using a semi- quantitative RT-PCR in liver, spleen and kidney tissues, which showed high expression after the bacterial infection indicating that A2M is immune responder against C. perfringens infection
Alpha 2-Macroglobulin Food- borne disease Zoonotic Disease