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Biological control of fungal blast disease in rice plants /

Farqad Fathi Hasan

Biological control of fungal blast disease in rice plants / المقاومة البٌيولوجٌية للفطرٌيات المسببة لمرض لفحة الورقة فى نبات الأرز Farqad Fathi Hasan ; Supervised Mary S. Khalil , Mohamed Hani Moubasher - Cairo : Farqad Fathi Hasan , 2015 - 152 P. : charts , facsimiles ; 25cm

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany and Microbiology

Fifty isolates of actinomycetes were isolated from Egyptian soil, rice field. All these isolates were identified according to (ISP) based on morphological and physiological characterization and found the fifty actinomycetes isolated belonged to eight Streptomyces species. Confirmed the results of morphological and physiological identification of isolate No.16 b according to molecular identification based of 16S sequences as Streptomyces flavotricini. All Streptomyces species were subjected for antagonistic test against rice blast fungus Pyricularia grisea. 50% of tested Streptomyces species did not reveal any antifungal activity against Pyricularia grisea, while 50% of tested organisms gave inhibition zone. The highest antifungal activities were obtained from the culture of Streptomyces flavotricini that gave inhibition zone (more than 40 mm), therefore this isolate was chosen for further investigations. The best conditions for the production of antifungal compounds were at 28C for 9 days, pH 7.2



Biological control Pyricularia grisea Streptomyces flavotricini