Mutagenesis and protoplast fusion of arthrobacter sp. for improved glucose isomerase production /
Heba Sayed Mohamad Mostafa
Mutagenesis and protoplast fusion of arthrobacter sp. for improved glucose isomerase production / التطفير و دمج البروتوبلاست لخلايا الأرثروباكتر لتحسين إنتاج الجلوكوز أيزوميريز Heba Sayed Mohamad Mostafa ; Supervised Sobhy Mohamed Mohsen , Wael Ahmed Bazaraa , Nagwa Mohamed Elshimi - Cairo : Heba Sayed Mohamad Mostafa , 2016 - 133 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Food Science and Technology
In attempts to construct superior glucose isomerase-producing strains, four strains were screened. Both Arthrobacter sp. and A. missouriensis were proved as the highest producers (16.8 and 15.6 U.ml-1, respectively). UV and Ethyl Methane Sulfonate (EMS) were used for mutagenesis. The mutant EMS 60-25 D generated from Arthrobacter sp. exhibited the highest GI production in this study (49.7 U.ml-1) with 3.2 folds improvement than its wild type. Protoplast fusion technology was successfully. The fusant (C 3-2) exhibited the highest GI synthesis (2.75 folds the wild type). The highest GI- producing fusant C 3-2 was used for the continuous production of HFCS. The maximal glucose conversion was 36% after 12h of isomerization at 60C
Glucose isomerase Mutation Protoplast fusion
Mutagenesis and protoplast fusion of arthrobacter sp. for improved glucose isomerase production / التطفير و دمج البروتوبلاست لخلايا الأرثروباكتر لتحسين إنتاج الجلوكوز أيزوميريز Heba Sayed Mohamad Mostafa ; Supervised Sobhy Mohamed Mohsen , Wael Ahmed Bazaraa , Nagwa Mohamed Elshimi - Cairo : Heba Sayed Mohamad Mostafa , 2016 - 133 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Food Science and Technology
In attempts to construct superior glucose isomerase-producing strains, four strains were screened. Both Arthrobacter sp. and A. missouriensis were proved as the highest producers (16.8 and 15.6 U.ml-1, respectively). UV and Ethyl Methane Sulfonate (EMS) were used for mutagenesis. The mutant EMS 60-25 D generated from Arthrobacter sp. exhibited the highest GI production in this study (49.7 U.ml-1) with 3.2 folds improvement than its wild type. Protoplast fusion technology was successfully. The fusant (C 3-2) exhibited the highest GI synthesis (2.75 folds the wild type). The highest GI- producing fusant C 3-2 was used for the continuous production of HFCS. The maximal glucose conversion was 36% after 12h of isomerization at 60C
Glucose isomerase Mutation Protoplast fusion