Molecular characterization between aflatoxinogenic and non- aflatoxinogenic strains of aspergillus flavus and aspergillus parasiticus /
Hadeel Mosfer Dakhil Aljaeed
Molecular characterization between aflatoxinogenic and non- aflatoxinogenic strains of aspergillus flavus and aspergillus parasiticus / التوصيف الجزيئي بين السلالات المنتجة والسلالات الغير منتجة لسموم الافالتوكسين فطري الاسبرجيلاس فلافس والاسبيرجيلس بارازيتيكس Hadeel Mosfer Dakhil Aljaeed ; Supervised Mohamed Hani Moubasher , Amira Abu Taleb - Cairo : Hadeel Mosfer Dakhil Aljaeed , 2016 - 88 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany and Microbiology
Not all A. flavus and A. parasiticus are aflatoxigenic. - Eight strains were aflatoxigenic and three were non aflatoxigenic out of eleven strains. Five strains of aflatoxinogenic fungi were A. flavus var. columnaris, two strains were A. parasiticus, and one strain was A. flavus. Three isolates were n o n-aflatoxinogenic. Two of which were A. flavus var columnaris and one A. flavus. - All aflatoxin-producing strains could release both aflatoxin B and G groups - Aflatoxin production was affected by broth type as shown in A. flavus var. columnaris strains 9022, 9024, 9025, 9030, 9031 A. flavus strain 9027 and A. parasiticus strains 9029, 9032. - In spite of, TLC distinguished between aflatoxin-producing and non-producing strains, it failed in some cases to detect aflatoxin- producing strains because instability of aflatoxin production may occur in certain toxinogenic strains. - Multiplex RT-PCR for the selected genes also showed a good correlation between aflatoxin gene expression and aflatoxin production. - Expression of nor-1 (Afl-D), omt- B (Afl-O), omt-A ((Afl-P)) and ord-A (Afl-Q) genes provided useful differentiation of aflatoxigenic and non- aflatoxigenic strains of A. flavus and A. parasiticus. - The expression of the selected genes varied with regard to the aflatoxin- producing ability and types of media
Aflatoxinogenic and non- aflatoxinogenic strains Aspergillus flavus and aspergillus parasiticus Molecular characterization
Molecular characterization between aflatoxinogenic and non- aflatoxinogenic strains of aspergillus flavus and aspergillus parasiticus / التوصيف الجزيئي بين السلالات المنتجة والسلالات الغير منتجة لسموم الافالتوكسين فطري الاسبرجيلاس فلافس والاسبيرجيلس بارازيتيكس Hadeel Mosfer Dakhil Aljaeed ; Supervised Mohamed Hani Moubasher , Amira Abu Taleb - Cairo : Hadeel Mosfer Dakhil Aljaeed , 2016 - 88 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany and Microbiology
Not all A. flavus and A. parasiticus are aflatoxigenic. - Eight strains were aflatoxigenic and three were non aflatoxigenic out of eleven strains. Five strains of aflatoxinogenic fungi were A. flavus var. columnaris, two strains were A. parasiticus, and one strain was A. flavus. Three isolates were n o n-aflatoxinogenic. Two of which were A. flavus var columnaris and one A. flavus. - All aflatoxin-producing strains could release both aflatoxin B and G groups - Aflatoxin production was affected by broth type as shown in A. flavus var. columnaris strains 9022, 9024, 9025, 9030, 9031 A. flavus strain 9027 and A. parasiticus strains 9029, 9032. - In spite of, TLC distinguished between aflatoxin-producing and non-producing strains, it failed in some cases to detect aflatoxin- producing strains because instability of aflatoxin production may occur in certain toxinogenic strains. - Multiplex RT-PCR for the selected genes also showed a good correlation between aflatoxin gene expression and aflatoxin production. - Expression of nor-1 (Afl-D), omt- B (Afl-O), omt-A ((Afl-P)) and ord-A (Afl-Q) genes provided useful differentiation of aflatoxigenic and non- aflatoxigenic strains of A. flavus and A. parasiticus. - The expression of the selected genes varied with regard to the aflatoxin- producing ability and types of media
Aflatoxinogenic and non- aflatoxinogenic strains Aspergillus flavus and aspergillus parasiticus Molecular characterization