Preparation of monoclonal antibodies against tetanus toxin and evaluation of its neutrelaizing potential /
Ahmed Adel Attia Seada
Preparation of monoclonal antibodies against tetanus toxin and evaluation of its neutrelaizing potential / تحضير اجسام مضادة وحيدة الفسيلة ضد سم التيتانوس وتقييم قدرتها المنا عية Ahmed Adel Attia Seada ; Supervised Rafik soliman , Nashwa Abdelsalam Ezz_Eldeen - Cairo : Ahmed adel attia seada , 2006 - 99P : ill ; 30cm
Thesis (M.Sc.) - Cairo University - Faculty Of Veterinary Medicine - Department Of Microbiology , Bacteriology , Mycology and Immunology
Preparation of monoclonal antibodies against tetanus toxin and evaluation of its efficacy obtained by using spleen cells (B - Cells) which harvested from well immunized BALB / c mice were used , also myeloma cell lines P3NS / 1 were usedFusion between spleen cells and myeloma cell lines occurred through fusogenic material called PEG 1500 , 40in hundred concentration to produce hybridomas which had the characteristic of both parents the ability to grow indefinitely from myeloma and the ability to produce of specific antibody from the B - cellsIn this study we succeed to produce five hybridomas producing monoclonal antibodies against tetanus toxin and also succeed to be cloned into 5 stable clones producing monoclonal antibodies against tetanus toxin
antibodies Monoclonal Tetanus toxin
Preparation of monoclonal antibodies against tetanus toxin and evaluation of its neutrelaizing potential / تحضير اجسام مضادة وحيدة الفسيلة ضد سم التيتانوس وتقييم قدرتها المنا عية Ahmed Adel Attia Seada ; Supervised Rafik soliman , Nashwa Abdelsalam Ezz_Eldeen - Cairo : Ahmed adel attia seada , 2006 - 99P : ill ; 30cm
Thesis (M.Sc.) - Cairo University - Faculty Of Veterinary Medicine - Department Of Microbiology , Bacteriology , Mycology and Immunology
Preparation of monoclonal antibodies against tetanus toxin and evaluation of its efficacy obtained by using spleen cells (B - Cells) which harvested from well immunized BALB / c mice were used , also myeloma cell lines P3NS / 1 were usedFusion between spleen cells and myeloma cell lines occurred through fusogenic material called PEG 1500 , 40in hundred concentration to produce hybridomas which had the characteristic of both parents the ability to grow indefinitely from myeloma and the ability to produce of specific antibody from the B - cellsIn this study we succeed to produce five hybridomas producing monoclonal antibodies against tetanus toxin and also succeed to be cloned into 5 stable clones producing monoclonal antibodies against tetanus toxin
antibodies Monoclonal Tetanus toxin