Detection and characterization of H.pylori in ruminants and Human contacts in Egypt /
Aya Abdallah Seleem
Detection and characterization of H.pylori in ruminants and Human contacts in Egypt / كشف وتوصيف هليكوباكتر بيلوري في الحيوانات المجترة والمخالطين لها من الآدميين في مصر Aya Abdallah Seleem ; Supervised Maha Ahmed Sabry , Khaled Abdelaziz Abdelmoein - Cairo : Aya Abdallah Seleem , 2016 - 100 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Zoonoses
Helicobacter pylori is considered as one of the most controversial bacteria in the world causing diverse gastrointestinal diseases in humans. The transmission way of this bacterium still remains obscure. Therefore, this study aimed to establish the roleof ruminants in transmission of this pathogen, as well as occurrence of H.pyloriinfection in humans and its associated risk factors. Out of 281 ruminants fecal samples (166 sheep, 46 goats and 34 cattle and 35 buffaloes)analysed, 34 (12.1%) were positive for Helicobacter species by PCR, through amplification of 16srRNA.H.pylori not detected inall positive samples examined for detection of cagA and glmMgenes by second PCR. DNA sequencing analysis of positive samples evidenced non-H.pylori species. Among these species H.canis, H.winghamensis,H.canadensisandH.equorum were identified in sheep. Meanwhile, H.canis and H.bovis were reported in goats and cattle respectively. Additionally, 16 stool specimens were collected from individuals contact with animalsand analysed by PCR sequencing, whereasH.canis was detected in 8.2% of animals and in 43.8% of humans.Moreover, 50 abomasal tissues were collected from both 30 sheep and 20 cattle that slaughtered in El-basateen abattoir in Cairo and evidenced H.bovissequencing insheep and cattle.Among the studied risk factors for H. pylori infection, the overall occurrence of H. pyloriIgG in human blood samples using ELISA technique was 41.6 % in symptomatic persons, meanwhile it was 30.3% in 10 asymptomatic persons.The highestantibodies positivity was detected in farmers
Helicobacter pylori Non H.pylori species Ruminants
Detection and characterization of H.pylori in ruminants and Human contacts in Egypt / كشف وتوصيف هليكوباكتر بيلوري في الحيوانات المجترة والمخالطين لها من الآدميين في مصر Aya Abdallah Seleem ; Supervised Maha Ahmed Sabry , Khaled Abdelaziz Abdelmoein - Cairo : Aya Abdallah Seleem , 2016 - 100 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Zoonoses
Helicobacter pylori is considered as one of the most controversial bacteria in the world causing diverse gastrointestinal diseases in humans. The transmission way of this bacterium still remains obscure. Therefore, this study aimed to establish the roleof ruminants in transmission of this pathogen, as well as occurrence of H.pyloriinfection in humans and its associated risk factors. Out of 281 ruminants fecal samples (166 sheep, 46 goats and 34 cattle and 35 buffaloes)analysed, 34 (12.1%) were positive for Helicobacter species by PCR, through amplification of 16srRNA.H.pylori not detected inall positive samples examined for detection of cagA and glmMgenes by second PCR. DNA sequencing analysis of positive samples evidenced non-H.pylori species. Among these species H.canis, H.winghamensis,H.canadensisandH.equorum were identified in sheep. Meanwhile, H.canis and H.bovis were reported in goats and cattle respectively. Additionally, 16 stool specimens were collected from individuals contact with animalsand analysed by PCR sequencing, whereasH.canis was detected in 8.2% of animals and in 43.8% of humans.Moreover, 50 abomasal tissues were collected from both 30 sheep and 20 cattle that slaughtered in El-basateen abattoir in Cairo and evidenced H.bovissequencing insheep and cattle.Among the studied risk factors for H. pylori infection, the overall occurrence of H. pyloriIgG in human blood samples using ELISA technique was 41.6 % in symptomatic persons, meanwhile it was 30.3% in 10 asymptomatic persons.The highestantibodies positivity was detected in farmers
Helicobacter pylori Non H.pylori species Ruminants