Phytochemical and biotechnological studies on diospyros kaki - family ebenaceae cultivated in Egypt /
Iman Abdelaziz Elseody Abdelgaffar Elsheikh
Phytochemical and biotechnological studies on diospyros kaki - family ebenaceae cultivated in Egypt / دراسات فيتوكيميائية و بيوتكنولوجية على نبات الديوسبيروس كاكى (العائلة الأبنوسية) المنزرع فى مصر Iman Abdelaziz Elseody Abdelgaffar Elsheikh ; Supervised Seham S. Elhawary , Soad Hanna Tadros , Medhat M. Seif Elnasr - Cairo : Iman Abdelaziz Elseody Abdelgaffar Elsheikh , 2016 - 317 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy
Diospyros kaki L. or costata cultivar is the main persimmon variety, progressively consumed in the Egyptian market and exportation, it is grown in clay soil under flood irrigation system. The volatile constituents isolated from leaves, comprised 6 identified components, constituting 83.12% of the total oil. GC/MS analysis of the unsaponifiable matter of fruits revealed the presence of 13 compounds, representing 85.61% of the total identified compounds, while that of leaves revealed the presence of 10 compounds, representing 87.16% of the total identified compounds. GC/MS analysis of the fatty acids methyl esters of fruits showed the presence of 13 components, representing 84.79% of the total identified compounds, while that leaves indicated the presence of 10 components, representing 91.07% of the total identified compounds. In addition, scopoletin, kaempferol, luteolin, rutin and apigenin 7-O-glucoside were isolated and identified by TLC, UV and 1H-NMR spectroscopic analysis. Biotechnological study showed that supplementation of MS medium with 10mg/l ZT+10 mg/ lIAA+500 mg / l PVP+0.1 mg / l Thiamine HCL recorded the best results of percentage of calli induction, and supplementation of 1/2 MS+1mg/l Zeatin+2 mg / lIAA+4 mg / l BA+0.5g / l PVP gave the best results of percentage of regenerated shootlets. The quantitative determination of the flavonoid and phenolic constituents of leaves and calli extracts (80% MeOH, each) using HPLC analysis, showed kaempferol (80.9 æg / g Dw) as the highest amount of flavonoid in leaves, and luteolin-6-arbinose 8-glucose (65.1 æg/g) in calli derived internode explants, while scopoletin was the highest phenolic compound recorded (57.08 and 25.3 æg/g) in both, respectively
Biotechnology Diospyros kaki L Phenolic compounds
Phytochemical and biotechnological studies on diospyros kaki - family ebenaceae cultivated in Egypt / دراسات فيتوكيميائية و بيوتكنولوجية على نبات الديوسبيروس كاكى (العائلة الأبنوسية) المنزرع فى مصر Iman Abdelaziz Elseody Abdelgaffar Elsheikh ; Supervised Seham S. Elhawary , Soad Hanna Tadros , Medhat M. Seif Elnasr - Cairo : Iman Abdelaziz Elseody Abdelgaffar Elsheikh , 2016 - 317 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacognosy
Diospyros kaki L. or costata cultivar is the main persimmon variety, progressively consumed in the Egyptian market and exportation, it is grown in clay soil under flood irrigation system. The volatile constituents isolated from leaves, comprised 6 identified components, constituting 83.12% of the total oil. GC/MS analysis of the unsaponifiable matter of fruits revealed the presence of 13 compounds, representing 85.61% of the total identified compounds, while that of leaves revealed the presence of 10 compounds, representing 87.16% of the total identified compounds. GC/MS analysis of the fatty acids methyl esters of fruits showed the presence of 13 components, representing 84.79% of the total identified compounds, while that leaves indicated the presence of 10 components, representing 91.07% of the total identified compounds. In addition, scopoletin, kaempferol, luteolin, rutin and apigenin 7-O-glucoside were isolated and identified by TLC, UV and 1H-NMR spectroscopic analysis. Biotechnological study showed that supplementation of MS medium with 10mg/l ZT+10 mg/ lIAA+500 mg / l PVP+0.1 mg / l Thiamine HCL recorded the best results of percentage of calli induction, and supplementation of 1/2 MS+1mg/l Zeatin+2 mg / lIAA+4 mg / l BA+0.5g / l PVP gave the best results of percentage of regenerated shootlets. The quantitative determination of the flavonoid and phenolic constituents of leaves and calli extracts (80% MeOH, each) using HPLC analysis, showed kaempferol (80.9 æg / g Dw) as the highest amount of flavonoid in leaves, and luteolin-6-arbinose 8-glucose (65.1 æg/g) in calli derived internode explants, while scopoletin was the highest phenolic compound recorded (57.08 and 25.3 æg/g) in both, respectively
Biotechnology Diospyros kaki L Phenolic compounds