Effect of some chemical mutagens on improving the micropropagated Banana plants to tolerate drought and salinity stresses /
Dalia Abdellatief Ibrahim Kishk
Effect of some chemical mutagens on improving the micropropagated Banana plants to tolerate drought and salinity stresses / بعض المطفرات الكيميائية على تحسين تحمل نباتات الموز المكثرة معملياً للإجهاد المائي والملحي Dalia Abdellatief Ibrahim Kishk ; Supervised Elsayed I. Gaber , Adel A. Abulsoad , Hattem M. Elshabrawi - Cairo : Dalia Abdellatief Ibrahim Kishk , 2017 - 213 P. : charts , facsmiles ; 25cm
Thesis (Ph.D.) - Cairo University - Institute of African Research and Studies - Department of Natural Resources
This study was carried out at the Tissue Culture Laboratory of the Horticulture Res. Inst. (HRI), Agricultural Research Center (ARC) and the Plant Biotechnology Dept., National Research Center (NRC) during the periods from 2012 till 2015. The aim of this study was to induce a tolerance mutant in banana plants for salt and drought stresses by using different chemical mutagenesis diethyl sulphate (DES) and sodium azide (NaN₃) and concentrations (100 oM for DES and 2 oM for NaN₃ expressed as low, 200 oM for DES and 4 oM for NaN₃ expressed as medium and 400 oM for DES and 8 oM for NaN₃ expressed as high) as well as soaking durations (10, 20 and 30 min) to increase its ability to tolerate salinity (500, 1000 and 1500 ppm) and drought stresses (PEG at 8, 16 and 32 gl⁻¹). Salinity and drought experiments were carried out during rooting stage and evaluated until the end of acclimatization stage. During multiplication stage, the results showed that NaN₃ was more effective in increasing number of shoots/plantlet but DES increased number of leaves and shoot length. High concentration for 30 min increased number of shoots and leaves to the highest values. Interaction between mutagen and concentrations plus duration revealed that DES concentrations produced the highest values except for number of shoots/plantlet which was more effective by using NaN₃ at 8 oM for 30 min. There were a great variance regarding the effect of mutagens, concentrations plus duration and studied stress (salinity and drought), individually and double interaction between these three factors during rooting and acclimatization stages. Treble interaction investigated that, DES at 100, 200 and 400 oM (to some extent) for different durations (10 to 30 min) could be used to reduce the negative effects of salinity (up to 1500 ppm) and drought (up to 32 g/l) during both rooting and acclimatization stages, with little exceptions toward NaN₃
Banana Grand Nain cv DES Diethyl sulphate
Effect of some chemical mutagens on improving the micropropagated Banana plants to tolerate drought and salinity stresses / بعض المطفرات الكيميائية على تحسين تحمل نباتات الموز المكثرة معملياً للإجهاد المائي والملحي Dalia Abdellatief Ibrahim Kishk ; Supervised Elsayed I. Gaber , Adel A. Abulsoad , Hattem M. Elshabrawi - Cairo : Dalia Abdellatief Ibrahim Kishk , 2017 - 213 P. : charts , facsmiles ; 25cm
Thesis (Ph.D.) - Cairo University - Institute of African Research and Studies - Department of Natural Resources
This study was carried out at the Tissue Culture Laboratory of the Horticulture Res. Inst. (HRI), Agricultural Research Center (ARC) and the Plant Biotechnology Dept., National Research Center (NRC) during the periods from 2012 till 2015. The aim of this study was to induce a tolerance mutant in banana plants for salt and drought stresses by using different chemical mutagenesis diethyl sulphate (DES) and sodium azide (NaN₃) and concentrations (100 oM for DES and 2 oM for NaN₃ expressed as low, 200 oM for DES and 4 oM for NaN₃ expressed as medium and 400 oM for DES and 8 oM for NaN₃ expressed as high) as well as soaking durations (10, 20 and 30 min) to increase its ability to tolerate salinity (500, 1000 and 1500 ppm) and drought stresses (PEG at 8, 16 and 32 gl⁻¹). Salinity and drought experiments were carried out during rooting stage and evaluated until the end of acclimatization stage. During multiplication stage, the results showed that NaN₃ was more effective in increasing number of shoots/plantlet but DES increased number of leaves and shoot length. High concentration for 30 min increased number of shoots and leaves to the highest values. Interaction between mutagen and concentrations plus duration revealed that DES concentrations produced the highest values except for number of shoots/plantlet which was more effective by using NaN₃ at 8 oM for 30 min. There were a great variance regarding the effect of mutagens, concentrations plus duration and studied stress (salinity and drought), individually and double interaction between these three factors during rooting and acclimatization stages. Treble interaction investigated that, DES at 100, 200 and 400 oM (to some extent) for different durations (10 to 30 min) could be used to reduce the negative effects of salinity (up to 1500 ppm) and drought (up to 32 g/l) during both rooting and acclimatization stages, with little exceptions toward NaN₃
Banana Grand Nain cv DES Diethyl sulphate