Biological study on the effect of transforming growth factors on hepatic somatic stem cells propagated ex vivo /
Mohammed Zaghloul Mohammed
Biological study on the effect of transforming growth factors on hepatic somatic stem cells propagated ex vivo / دراسة بيولوجية على تأثيرعوامل النموعلى الخلايا الجذعية الجسدية الكبدية المعزولة من مرضى سرطان الكبد Mohammed Zaghloul Mohammed ; Supervised Motawa E. Elhussieni , Alaa Eldean Ismael A. Almottleb , Ahmad H. Elhabashy - Cairo : Mohammed Zaghloul Mohammed , 2018 - 159 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Cancer Biology
Objective: This study had attempted to study the effect of TGF-Ý1 on hepatic somatic stem cells (HSSC) in vitro, since it could play an active role in the process of hepatocarcinogenesis and cancer metastases. We aimed to determine the HCC-specific molecular mechanisms mediating TGF-Ý1 dysregulation to a driver of tumor progression and characterize the molecular network between TGF-Ý1 and c-KIT signaling. Methods: Cancer stem cells were isolated from Hepatocellular carcinoma patients of different grades, and from age and sex matched healthy controls from safety margin and R N A isolation was done to get PCR product for mutations detection. Results: The results of this study showed that by using monoclonal characterization CD44 and CD90 for non-malignant liver tissue origin cells there are significant decreasing in CD40+CD90+ %by increasing TGF Ý1 concentration (P value <0.001). while for malignant liver tissue origin cells there are significant decreasing in CD40+CD90+ % only by using TGF Ý1 at concentration2.5 and 5 ng/dl P value <0.001
Cancer stem cells Hepatocellular carcinoma TGF Ý1 and C-kit gene
Biological study on the effect of transforming growth factors on hepatic somatic stem cells propagated ex vivo / دراسة بيولوجية على تأثيرعوامل النموعلى الخلايا الجذعية الجسدية الكبدية المعزولة من مرضى سرطان الكبد Mohammed Zaghloul Mohammed ; Supervised Motawa E. Elhussieni , Alaa Eldean Ismael A. Almottleb , Ahmad H. Elhabashy - Cairo : Mohammed Zaghloul Mohammed , 2018 - 159 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Cancer Biology
Objective: This study had attempted to study the effect of TGF-Ý1 on hepatic somatic stem cells (HSSC) in vitro, since it could play an active role in the process of hepatocarcinogenesis and cancer metastases. We aimed to determine the HCC-specific molecular mechanisms mediating TGF-Ý1 dysregulation to a driver of tumor progression and characterize the molecular network between TGF-Ý1 and c-KIT signaling. Methods: Cancer stem cells were isolated from Hepatocellular carcinoma patients of different grades, and from age and sex matched healthy controls from safety margin and R N A isolation was done to get PCR product for mutations detection. Results: The results of this study showed that by using monoclonal characterization CD44 and CD90 for non-malignant liver tissue origin cells there are significant decreasing in CD40+CD90+ %by increasing TGF Ý1 concentration (P value <0.001). while for malignant liver tissue origin cells there are significant decreasing in CD40+CD90+ % only by using TGF Ý1 at concentration2.5 and 5 ng/dl P value <0.001
Cancer stem cells Hepatocellular carcinoma TGF Ý1 and C-kit gene