Assessment of the role of NOGO gene block in enhancing the neuro-regenerative effect of mesenchymal stem cells /
Eman Ahmed Youssef
Assessment of the role of NOGO gene block in enhancing the neuro-regenerative effect of mesenchymal stem cells / في تعزيز تأثير التجدد العصبي للخلايا الجذعية الوسيطة NOGO تقييم دور إسكات كتلة الجين Eman Ahmed Youssef ; Supervised Hala Gabr , Reham Afeefy , Nirmeen Adel - Cairo : Eman Ahmed Youssef , 2018 - 238 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology
Aim: Amyotrophic Lateral Sclerosis (ALS) is a fatal disease. Mesenchymal stem cells (MSCs) have a therapeutic potential in neuronal diseases, factors as NOGO A limit the axonal regeneration. In this study, gene silencing was used to suppress Nogo-A expression in MSCs, augmenting their neuro-regenerative role. Methods: MSCs were cultured from mice bone marrow then transfected with a plasmid coding for NOGO-A gene SiRNA, mouse models of motor neuron degeneration were created, therapeutic role of non-modified and plasmid transfected MSCs were studied and assessed clinically and histologically. Group 1 mice received no treatment. Group 2 received an IV injection of non modified MSCs and sacrificed 8 days (subgroup 2a) and 15 days (subgroup 2b) following treatment, Group 3 received an IV injection of transfected MSCs and were sacrificed 8 days (subgroup 3a) and 15 days (subgroup 3b) following treatment
Amyotrophic Lateral Sclerosis (ALS) Mesenchymal stem cells (MSCs) Neuroregeneration
Assessment of the role of NOGO gene block in enhancing the neuro-regenerative effect of mesenchymal stem cells / في تعزيز تأثير التجدد العصبي للخلايا الجذعية الوسيطة NOGO تقييم دور إسكات كتلة الجين Eman Ahmed Youssef ; Supervised Hala Gabr , Reham Afeefy , Nirmeen Adel - Cairo : Eman Ahmed Youssef , 2018 - 238 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology
Aim: Amyotrophic Lateral Sclerosis (ALS) is a fatal disease. Mesenchymal stem cells (MSCs) have a therapeutic potential in neuronal diseases, factors as NOGO A limit the axonal regeneration. In this study, gene silencing was used to suppress Nogo-A expression in MSCs, augmenting their neuro-regenerative role. Methods: MSCs were cultured from mice bone marrow then transfected with a plasmid coding for NOGO-A gene SiRNA, mouse models of motor neuron degeneration were created, therapeutic role of non-modified and plasmid transfected MSCs were studied and assessed clinically and histologically. Group 1 mice received no treatment. Group 2 received an IV injection of non modified MSCs and sacrificed 8 days (subgroup 2a) and 15 days (subgroup 2b) following treatment, Group 3 received an IV injection of transfected MSCs and were sacrificed 8 days (subgroup 3a) and 15 days (subgroup 3b) following treatment
Amyotrophic Lateral Sclerosis (ALS) Mesenchymal stem cells (MSCs) Neuroregeneration