Detection of carbapenemase producing Enterobacteriaceae using the modified carbapenem inactivation method /
Hagar Lotfy Abdelbaky Mowafy
Detection of carbapenemase producing Enterobacteriaceae using the modified carbapenem inactivation method / الكشف عن البكتريا المعوية المنتجة للكاربابنيميز باستخدام طريقة تعطيل الكاربابينيم المعدلة Hagar Lotfy Abdelbaky Mowafy ; Supervised Mohammad Amr Elkholy , Maha Mahmoud Kotb - Cairo : Hagar Lotfy Abdelbaky Mowafy , 2018 - 88 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology
Carbapenems are a class of beta (Ý)-lactam antibiotics with broad spectrum of activity. They are often considered as a last resort in treatment of infections caused by multidrug resistant organisms. Carbapenem resistant Enterobacteriaceae (CRE) have been reported worldwide. Resistance to carbapenems in Enterobacteriaceae is caused mainly by carbapenemase production or by porin loss combined with the expression of Ý-lactamases like extended-spectrum Ý-lactamases (ESBL) or ampicillin class C (AmpC). In the present study, we attempted to determine the prevalence of carbapenemase-producing Enterobacteriaceae (CPE) among 202 clinical isolates of Enterobacteriaceae by the phenotypic test modified carbapenem inactivation method (mCIM) Initial screening for carbapenemase- producing isolates among the 202 Enterobacteriaceae isolates was done by minimum inhibitory concentration (MIC) determination for ertapenem by broth microdilution method. Confirmation of carbapenemase production among ertapenem-resistant isolates was done by the phenotypic test mCIM. The results of the current study showed that the prevalence of CRE by broth microdilution method was 36.1% and the prevalence of CPE among resistant isolates was 80.8% by mCIM
Carbapenemases producing Enterobacteriaceae Enterobacteriaceae Microdilution method
Detection of carbapenemase producing Enterobacteriaceae using the modified carbapenem inactivation method / الكشف عن البكتريا المعوية المنتجة للكاربابنيميز باستخدام طريقة تعطيل الكاربابينيم المعدلة Hagar Lotfy Abdelbaky Mowafy ; Supervised Mohammad Amr Elkholy , Maha Mahmoud Kotb - Cairo : Hagar Lotfy Abdelbaky Mowafy , 2018 - 88 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology
Carbapenems are a class of beta (Ý)-lactam antibiotics with broad spectrum of activity. They are often considered as a last resort in treatment of infections caused by multidrug resistant organisms. Carbapenem resistant Enterobacteriaceae (CRE) have been reported worldwide. Resistance to carbapenems in Enterobacteriaceae is caused mainly by carbapenemase production or by porin loss combined with the expression of Ý-lactamases like extended-spectrum Ý-lactamases (ESBL) or ampicillin class C (AmpC). In the present study, we attempted to determine the prevalence of carbapenemase-producing Enterobacteriaceae (CPE) among 202 clinical isolates of Enterobacteriaceae by the phenotypic test modified carbapenem inactivation method (mCIM) Initial screening for carbapenemase- producing isolates among the 202 Enterobacteriaceae isolates was done by minimum inhibitory concentration (MIC) determination for ertapenem by broth microdilution method. Confirmation of carbapenemase production among ertapenem-resistant isolates was done by the phenotypic test mCIM. The results of the current study showed that the prevalence of CRE by broth microdilution method was 36.1% and the prevalence of CPE among resistant isolates was 80.8% by mCIM
Carbapenemases producing Enterobacteriaceae Enterobacteriaceae Microdilution method