In vitro study on the propagation , conservation and DNA of Odontonema cuspidatum (Nees) O.Ktze Plant /
Asmaa Elsayed Abdelhafez
In vitro study on the propagation , conservation and DNA of Odontonema cuspidatum (Nees) O.Ktze Plant / Odontonema cuspidatum دراسة معملية على الاكثار و الحفظ و الحمض النووى لنبات Asmaa Elsayed Abdelhafez ; Supervised Salwa Salem Sakr , Mohamed Abdelkhalek Elkhateeb , Mamdouh Ahmed Elshamy - Cairo : Asmaa Elsayed Abdelhafez , 2018 - 155 P. : charts ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Ornamental Horticulture
This investigation was carried out during the period from 2014 to 2017. Using NaOCl at 2% for 25 min was the most effective. Culturing of the explants on full MS medium supplemented with 0.5 mg/l TDZ, was positively, 1mg/l IBA and 2 mg/l NAA resulted in the highest number of roots. peat moss+ sand at the ratio of 3:1 (v/v) to acclimatization. For encapsulation used sodium alginate (5 %) and 1.1 g/100ml CaCl2.2H2o2. Using sucrose at 50 g/l as osmotic agent was the best in all storage periods. RAPD- based DNA fingerprints gave no eridence of on variation during in vitro conservation
In vitro Micropropagation Tissue culture
In vitro study on the propagation , conservation and DNA of Odontonema cuspidatum (Nees) O.Ktze Plant / Odontonema cuspidatum دراسة معملية على الاكثار و الحفظ و الحمض النووى لنبات Asmaa Elsayed Abdelhafez ; Supervised Salwa Salem Sakr , Mohamed Abdelkhalek Elkhateeb , Mamdouh Ahmed Elshamy - Cairo : Asmaa Elsayed Abdelhafez , 2018 - 155 P. : charts ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Ornamental Horticulture
This investigation was carried out during the period from 2014 to 2017. Using NaOCl at 2% for 25 min was the most effective. Culturing of the explants on full MS medium supplemented with 0.5 mg/l TDZ, was positively, 1mg/l IBA and 2 mg/l NAA resulted in the highest number of roots. peat moss+ sand at the ratio of 3:1 (v/v) to acclimatization. For encapsulation used sodium alginate (5 %) and 1.1 g/100ml CaCl2.2H2o2. Using sucrose at 50 g/l as osmotic agent was the best in all storage periods. RAPD- based DNA fingerprints gave no eridence of on variation during in vitro conservation
In vitro Micropropagation Tissue culture