Molecular characterization of infectious bursal disease virus in some Egyptian governorates in 2015 /
Aml Saad Ahmed Elsaadany
Molecular characterization of infectious bursal disease virus in some Egyptian governorates in 2015 / التوصيف الجزيئى لفيروس التهاب غدة فابريسى المعدى فى بعض محافظات مصر فى 2015 Aml Saad Ahmed Elsaadany ; Supervised Hussien Aly Hussien Ahmed , Ayman Hany Mahmoud Eldeeb - Cairo : Aml Saad Ahmed Elsaadany , 2019 - 85 P. : charts , maps ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology
Infectious bursal disease virus remerged frequently in Egypt, causing serious economic losses. The disease is acute contagious disease in young chickens from 3-6 weeks of age. In present study forty field bursa samples were collected from different Egyptian governorates, detection of IBDV in the collected samples were done using RT-PCR. Eight samples showed positive results by percent of (20%). Sequence analysis and characterization of the hyper variable region of VP2 gene from four strains purified from the PCR product showed many amino acid substitutions that may affect IBDV antigenicity and virulence. Comparative phylogenetic analysis based on the sequence of the hypervariable region of VP2 gene show that the strains were closer to Giza 2008 strains. This study reports the continuous circulation of vvIBDV strains in vaccinated flocks 4 governorates 2Giza, Sharkia, Behira and Dakahlia3, in Egypt during year 2015. There are need to review the control and vaccination strategy of IBDV in broiler flocks specially the extensive abuse of using classical vaccines
Bursal disease virus IBDV VP2
Molecular characterization of infectious bursal disease virus in some Egyptian governorates in 2015 / التوصيف الجزيئى لفيروس التهاب غدة فابريسى المعدى فى بعض محافظات مصر فى 2015 Aml Saad Ahmed Elsaadany ; Supervised Hussien Aly Hussien Ahmed , Ayman Hany Mahmoud Eldeeb - Cairo : Aml Saad Ahmed Elsaadany , 2019 - 85 P. : charts , maps ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology
Infectious bursal disease virus remerged frequently in Egypt, causing serious economic losses. The disease is acute contagious disease in young chickens from 3-6 weeks of age. In present study forty field bursa samples were collected from different Egyptian governorates, detection of IBDV in the collected samples were done using RT-PCR. Eight samples showed positive results by percent of (20%). Sequence analysis and characterization of the hyper variable region of VP2 gene from four strains purified from the PCR product showed many amino acid substitutions that may affect IBDV antigenicity and virulence. Comparative phylogenetic analysis based on the sequence of the hypervariable region of VP2 gene show that the strains were closer to Giza 2008 strains. This study reports the continuous circulation of vvIBDV strains in vaccinated flocks 4 governorates 2Giza, Sharkia, Behira and Dakahlia3, in Egypt during year 2015. There are need to review the control and vaccination strategy of IBDV in broiler flocks specially the extensive abuse of using classical vaccines
Bursal disease virus IBDV VP2