Biological evaluation of Interleukines 2, 12 and taurine combination against human hepatoma cells propagated ex-vivo /
Tareq Hassan Muhmmad Jorob
Biological evaluation of Interleukines 2, 12 and taurine combination against human hepatoma cells propagated ex-vivo / التقييم البيولوجى للإنترلوكين 2, 12 و التورين المكمل للتأثير على خلايا أورام الكبد الخبيثة و التى تنمو خارج الجسم Tareq Hassan Muhmmad Jorob ; Supervised Motawa E. Elhouseini , Saad M. Elgendy , Abdelbary Prince - Cairo : Tareq Hassan Muhmmad Jorob , 2019 - 97 P. : charts ; 25cm
Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Cancer Biology
Hepatocellular carcinoma (HCC) is the sixth most prevalent malignancy worldwide and is a rising cause of cancer related mortality. Therefore, it is important to search for agents that have a potential anticancer effect against HCC. The objective of the present study was to investigate the anti-cancer effects of interlekin-2 (IL-2), IL-12 and taurine in human hepatocellular carcinoma cells (Hep-G2). HepG2 cells was obtained from the National Cancer Institute and cultured in RPMI medium. Cells were classified into 6 groups, HepG2 cells without treatment as control group (group A), HepG2 cells treated with IL-2 (group B), HepG2 cells treated with IL-12 (group C), HepG2 cells treated with taurine (group D), HepG2 cells treated with IL-2 and IL-12 (group E) and HepG2 cells treated with IL-2, IL-12 and Taurine (group F). Viability of HepG2 cells was evaluated using MTT assay. Group B, E and F were significantly different from the control group with decreased viability in contrast to group C and D. LDH release assay was used to measure cytotoxicity of the previously mentioned natural agents. There was no significant difference between group D and the control group, while apparent cytotoxicity was observed in group B, C, E and F
Hepatocellular carcinoma (HCC) Interleukines 2,12 Taurine combination
Biological evaluation of Interleukines 2, 12 and taurine combination against human hepatoma cells propagated ex-vivo / التقييم البيولوجى للإنترلوكين 2, 12 و التورين المكمل للتأثير على خلايا أورام الكبد الخبيثة و التى تنمو خارج الجسم Tareq Hassan Muhmmad Jorob ; Supervised Motawa E. Elhouseini , Saad M. Elgendy , Abdelbary Prince - Cairo : Tareq Hassan Muhmmad Jorob , 2019 - 97 P. : charts ; 25cm
Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Cancer Biology
Hepatocellular carcinoma (HCC) is the sixth most prevalent malignancy worldwide and is a rising cause of cancer related mortality. Therefore, it is important to search for agents that have a potential anticancer effect against HCC. The objective of the present study was to investigate the anti-cancer effects of interlekin-2 (IL-2), IL-12 and taurine in human hepatocellular carcinoma cells (Hep-G2). HepG2 cells was obtained from the National Cancer Institute and cultured in RPMI medium. Cells were classified into 6 groups, HepG2 cells without treatment as control group (group A), HepG2 cells treated with IL-2 (group B), HepG2 cells treated with IL-12 (group C), HepG2 cells treated with taurine (group D), HepG2 cells treated with IL-2 and IL-12 (group E) and HepG2 cells treated with IL-2, IL-12 and Taurine (group F). Viability of HepG2 cells was evaluated using MTT assay. Group B, E and F were significantly different from the control group with decreased viability in contrast to group C and D. LDH release assay was used to measure cytotoxicity of the previously mentioned natural agents. There was no significant difference between group D and the control group, while apparent cytotoxicity was observed in group B, C, E and F
Hepatocellular carcinoma (HCC) Interleukines 2,12 Taurine combination