Influence of laser photobiomodulation and biodentine on proliferation and odontogenic differentiation of human dental pulp mesenchymal stem cells : In-vitro study /
Mustafa Najah Hasan Aldarraji
Influence of laser photobiomodulation and biodentine on proliferation and odontogenic differentiation of human dental pulp mesenchymal stem cells : In-vitro study / تأثير التحفيز الحيوى الضوئى بالليزر مع مادة البايودنتين على الانتشار والتمايز السني للخلايا الجذعية اللبية السنية : دراسة داخل المختبر Mustafa Najah Hasan Aldarraji ; Supervised Latifa Mohamed Abdelgawad , Dina Sabry Abdelfatah - Cairo : Mustafa Najah Hasan Aldarraji , 2020 - 109 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Sciences (NILES) - Department of Laser Application in Oral Surgery
Background: Vital pulp therapy preserves the health of injured dental pulp tissues through improving cells viability and formation of reparative dentine. Objective: Present study was conducted to assess the odontogenic effects of Biodentine (BD) and 650nm laser Photobiomodulation (PBM) in dentine regeneration from human dental pulp stem cells (HDPSCs). Methods: Seventy-two HDPSCs well-plates were divided into the following: Group1: control. Group2: treated by BD. Group3: treated by 650nm PBM with 1J/cm2. Group4: treated by both 650nm PBM with 1J/cm2and BD. Group5: treated by 650nm PBM with 2J/cm2. Group6: treated by both 650nm PBM with 2J/cm2and BD. Cell viability was measured by MTT assay for 4days. While odontogenic differentiation of HDPSCs was assessed by alkaline-phosphatase-activity (ALP), Von kossa stain (VKS), and Reverse-transcriptase-PCR (RT-PCR) for dentine-sialophosphoprotein (DSPP), Runt-related-transcription-factor2 (RUNX2) Bone morphogenic protein type 2 (BMP2) and collagen type I (COL I) at day 21. Results: The results showed statistically significant increase in viability of all study groups against control (p-value <0.0001) at 96 hours. Also showed significant differences in ALP activity between groups (p-value <0.0001) and in expression of DSPP, RUNX2, BMP2 and COL I (P<0.001) compared to control. The combination of both BD and PBM 2J/cm2 showed the highest number of calcified nodules
Biodentine Human dental pulp stem cells (HDPSCs) Photobiomodulation
Influence of laser photobiomodulation and biodentine on proliferation and odontogenic differentiation of human dental pulp mesenchymal stem cells : In-vitro study / تأثير التحفيز الحيوى الضوئى بالليزر مع مادة البايودنتين على الانتشار والتمايز السني للخلايا الجذعية اللبية السنية : دراسة داخل المختبر Mustafa Najah Hasan Aldarraji ; Supervised Latifa Mohamed Abdelgawad , Dina Sabry Abdelfatah - Cairo : Mustafa Najah Hasan Aldarraji , 2020 - 109 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Sciences (NILES) - Department of Laser Application in Oral Surgery
Background: Vital pulp therapy preserves the health of injured dental pulp tissues through improving cells viability and formation of reparative dentine. Objective: Present study was conducted to assess the odontogenic effects of Biodentine (BD) and 650nm laser Photobiomodulation (PBM) in dentine regeneration from human dental pulp stem cells (HDPSCs). Methods: Seventy-two HDPSCs well-plates were divided into the following: Group1: control. Group2: treated by BD. Group3: treated by 650nm PBM with 1J/cm2. Group4: treated by both 650nm PBM with 1J/cm2and BD. Group5: treated by 650nm PBM with 2J/cm2. Group6: treated by both 650nm PBM with 2J/cm2and BD. Cell viability was measured by MTT assay for 4days. While odontogenic differentiation of HDPSCs was assessed by alkaline-phosphatase-activity (ALP), Von kossa stain (VKS), and Reverse-transcriptase-PCR (RT-PCR) for dentine-sialophosphoprotein (DSPP), Runt-related-transcription-factor2 (RUNX2) Bone morphogenic protein type 2 (BMP2) and collagen type I (COL I) at day 21. Results: The results showed statistically significant increase in viability of all study groups against control (p-value <0.0001) at 96 hours. Also showed significant differences in ALP activity between groups (p-value <0.0001) and in expression of DSPP, RUNX2, BMP2 and COL I (P<0.001) compared to control. The combination of both BD and PBM 2J/cm2 showed the highest number of calcified nodules
Biodentine Human dental pulp stem cells (HDPSCs) Photobiomodulation