Microbiological and molecular studies on Helicobacter species in equine /
Dina AbdElwahab Ezzat Hussien Abuelghait
Microbiological and molecular studies on Helicobacter species in equine / دراسات ميكروبيولوجية وجزيئية على ميكروب الهليكوباكتر فى الفصيلة الخيلية Dina AbdElwahab Ezzat Hussien Abuelghait ; Supervised Khaled Farouk Elamry , Eldessouky Mohammed Sheta , Ahmed Samir Mohammed - Cairo : Dina AbdElwahab Ezzat Hussien Abuelghait , 2021 - 115 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
The aim of the present study is to make microbiological and molecular studies on helicobactersin Equine.Helicobacter pylori is one of the most common human pathogens worldwide with serious clinical out-comes, although several risk factors for the development of equine gastric ulcers had been widely studied, investigation of microbiological factors has been limited, therefore in our study, fifty gastric lavage and fecal samples were collected from fifty diseased horses and application of Lateral Flow test for antigen detection and PCR assays for detection of Helicobacter species. Positive results confirmed only by PCR which revealed that twelve samples were positive among the examined samples (24%), sequencing of 16s rRNA gene amplicon shared 100% identity with H.equorum on GenBank, young foals show higher prevalence than adult horses. Occasionaly, H.equorum act as a potential zoonotic risk hazard thus, further studies are needed to investigate the zoonotic pathway to the human contacts
Equine Helicobacterspecies Molecular
Microbiological and molecular studies on Helicobacter species in equine / دراسات ميكروبيولوجية وجزيئية على ميكروب الهليكوباكتر فى الفصيلة الخيلية Dina AbdElwahab Ezzat Hussien Abuelghait ; Supervised Khaled Farouk Elamry , Eldessouky Mohammed Sheta , Ahmed Samir Mohammed - Cairo : Dina AbdElwahab Ezzat Hussien Abuelghait , 2021 - 115 P. : charts , facsimiles ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
The aim of the present study is to make microbiological and molecular studies on helicobactersin Equine.Helicobacter pylori is one of the most common human pathogens worldwide with serious clinical out-comes, although several risk factors for the development of equine gastric ulcers had been widely studied, investigation of microbiological factors has been limited, therefore in our study, fifty gastric lavage and fecal samples were collected from fifty diseased horses and application of Lateral Flow test for antigen detection and PCR assays for detection of Helicobacter species. Positive results confirmed only by PCR which revealed that twelve samples were positive among the examined samples (24%), sequencing of 16s rRNA gene amplicon shared 100% identity with H.equorum on GenBank, young foals show higher prevalence than adult horses. Occasionaly, H.equorum act as a potential zoonotic risk hazard thus, further studies are needed to investigate the zoonotic pathway to the human contacts
Equine Helicobacterspecies Molecular