Analytical studies and bio control of ochratoxin A in wheat /
Amira Ahmed Abdelrahman Abdelhafez
Analytical studies and bio control of ochratoxin A in wheat / في القمح A دراسات تحليلية وتحكم حيوي للأوكراتوكسين Amira Ahmed Abdelrahman Abdelhafez ; Supervised Faten Nour Eldien , Ahmed Mamdouh Ibrahim , Sally Elsayed Ahmed Elashry - Cairo : Amira Ahmed Abdelrahman Abdelhafez , 2021 - 72 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Analytical Chemistry
Ochratoxin A (OTA) has been considered a carcinogenic, teratogenic, hepatotoxic, and nephrotoxic thus reduction of OTA considered of high priority besides its economic effect on vets trading field. A Method for the determination of OTA by HPLC-FLD has been adopted and validated.The method recoveries were (89-97%) while the limit of detection (LOD) and limit of quantification (LOQ) were (0.05-1 og/kg), respectively.The study has concluded the efficiency and selectivity of IAC for OTA determination in wheat samples under proper selected conditions. The method is internationally accredited by FINAS for fulfilling the requirements of ISO/IEC 17025/2017 standard. Bio control is considered one of the most promising and more safety strategies. Five organisms were examined for activities against OTA producing Aspergillus Steynii, Trichoderma harzianum,Trichoderma viride, and Bacillus mycodis had no antifungal effect. B. subtilus and P. aeruginosa have affected the colony. The growth rate of A. steynii had been affected by different bacterial extract concentrations of both B. subtilus and P. aeruginosa on YES media. The OTA levels had been produced by A. styenii affected by different chloroform extract concentrations of both B. subtilus and P. aeruginosa on YES media and stored wheat
LOD LOQ OTA
Analytical studies and bio control of ochratoxin A in wheat / في القمح A دراسات تحليلية وتحكم حيوي للأوكراتوكسين Amira Ahmed Abdelrahman Abdelhafez ; Supervised Faten Nour Eldien , Ahmed Mamdouh Ibrahim , Sally Elsayed Ahmed Elashry - Cairo : Amira Ahmed Abdelrahman Abdelhafez , 2021 - 72 P. : charts , facsimiles ; 25cm
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Analytical Chemistry
Ochratoxin A (OTA) has been considered a carcinogenic, teratogenic, hepatotoxic, and nephrotoxic thus reduction of OTA considered of high priority besides its economic effect on vets trading field. A Method for the determination of OTA by HPLC-FLD has been adopted and validated.The method recoveries were (89-97%) while the limit of detection (LOD) and limit of quantification (LOQ) were (0.05-1 og/kg), respectively.The study has concluded the efficiency and selectivity of IAC for OTA determination in wheat samples under proper selected conditions. The method is internationally accredited by FINAS for fulfilling the requirements of ISO/IEC 17025/2017 standard. Bio control is considered one of the most promising and more safety strategies. Five organisms were examined for activities against OTA producing Aspergillus Steynii, Trichoderma harzianum,Trichoderma viride, and Bacillus mycodis had no antifungal effect. B. subtilus and P. aeruginosa have affected the colony. The growth rate of A. steynii had been affected by different bacterial extract concentrations of both B. subtilus and P. aeruginosa on YES media. The OTA levels had been produced by A. styenii affected by different chloroform extract concentrations of both B. subtilus and P. aeruginosa on YES media and stored wheat
LOD LOQ OTA