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Assessment the efficacy of erythrosine photosensitizer on periodontal pathogens /

Eman Mohamed Abdelgawad Shaheen

Assessment the efficacy of erythrosine photosensitizer on periodontal pathogens / تقييم تأثير الايرثروسين كمحفز ضوئى على بكتيريا الجيوب اللثوية Eman Mohamed Abdelgawad Shaheen ; Supervised Aly M. Elhusseiny Saafan , Latifa Abdelgawad , Somaia Abdellateef Eissa - Cairo : Eman Mohamed Abdelgawad Shaheen , 2020 - 72 P. : charts ; 25cm

Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Sciences (NILES) - Department of Laser Applications in Medical Biological

Objectives: Photodynamic therapy is an alternative therapeutic approach for eradicating pathogenic bacteria in periodontal diseases. As the conventional treatment doesnt completely eliminate periodontal pathogens. For this purpose, the aim of this study was to evaluate the efficacy of erythrosine photosensitizer with light emitting diode LED or diode laser on Fusobacterium nucleatum as periodontal pathogens. Material and methods: Standard suspension of F. nucleatum were exposed to Light Emitting Diode (440-480 nm) or green laser (532 nm) in combination with erythrosine (22 om) for 2, 3 and 5 min irradiation time. using identical irradiation parameters for comparison between treatment groups ( control untreated photosensitizer only blue light only green laser only blue light with erythrosine green laser with erythrosine ). Survival of bacteria was determined by counting the number of colony forming units (CFU) after incubation. The results were tabulated and statistically analyzed. Results: Erythrosine in combination with (blue LED or green laser) at exposure time 5 min showed the highest bacterial reduction (98 %) with a significant difference than erythrosine alone or light / laser alone (p = 000.1)



Erythrosine Erythrosine photosensitizer Periodontal pathogens