MARC details
| 000 -LEADER |
|---|
| fixed length control field |
06435namaa22004331i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20250223033207.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
231108s2022 ua a|||frm||| 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GICUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GICUC |
| Modifying agency |
EG-GICUC |
| Description conventions |
rda |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| Language code of summary or abstract |
eng |
| -- |
ara |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposit |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
633.112 |
| Edition number |
21 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) |
|---|
| Classification number |
633.112 |
| Edition number |
21 |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.12.25.M.Sc.2022.Ha.D |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Hamiss Ali Mohamed Abul Ela, |
| Relator term |
preparation. |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Development of abiotic stress tolerant wheat plants via gene editing engineering / |
| Statement of responsibility, etc. |
by Hamiss Ali Mohamed Abul Ela ; supervision Prof.Dr. Ezz Eldein Shafei, Dr. Mahmoud Saleh Mahmoud. |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
تطوير نباتات القمح التى تتحمل الاجهاد الغير حيوي من خلال هندسة تحرير الجينات |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Date of production, publication, distribution, manufacture, or copyright notice |
2022. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
120 pages : |
| Other physical details |
Illustrations ; |
| Dimensions |
25 cm. + |
| Accompanying material |
CD. |
| 336 ## - CONTENT TYPE |
|---|
| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
|---|
| Source |
rdamedia |
| Media type term |
Unmediated |
| 338 ## - CARRIER TYPE |
|---|
| Source |
rdacarrier |
| Carrier type term |
volume |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (M.Sc.)-Cairo University, 2022. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Bibliography: pages 110-118. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
As a consequence of the ongoing global climate changes, low water<br/>availability and salinization are expected to affect up to 50% of all arable lands<br/>by the year 2050. Wheat is one of the most consumed crops all over the world,<br/>especially in Egypt. Therefore, improving the tolerance of wheat to drought<br/>and salinity stresses which are correlated (abiotic stress) is vital to ensure food<br/>security. Lately, the CRISPR/Cas9 (Clustered Regularly Interspaced Short<br/>Palindromic Repeats/CRISPR associated nuclease 9) system has been used as<br/>a powerful tool for improving traits with high efficiency. So, this study aimed<br/>for using the CRISPR/Cas9 system to knock out the SALI gene (which down-<br/>regulation increases drought tolerance in plants other than wheat) in a bread<br/>wheat cultivar to increase its drought and salinity tolerance. Marker-assisted<br/>selection using 9SSR primer pairs, 43 SCoT primers, and 42 combinations of<br/>SRAP primers (7 Me and 6 Em) along with biochemical analysis of K+/Na+<br/>ratio on 10 of the most cultivated bread wheat cultivars in Egypt (Giza 171,<br/>Giza 168, Misr 1, Misr 2, Sids 11, Sids 12, Gemaiza 9, Gemaiza 11, Gemaiza<br/>12, and Shandaweel 1) revealed that Giza 171, Gemaiza 9, and Gemaiza 11<br/>are genetically similar sharing the same ancestor and also Giza 171 and<br/>Gemaiza 11 showed the lowest K+/Na+ ratio and high Na+ content, so they<br/>are considered non-tolerant under high salt concentrations. Construct “2000”<br/>containing 3gRNAs targeting 3 different exons on all copies of wheat’s SALI<br/>gene were used to induce mutations in Giza 171 to induce stomatal closure,<br/>thus increasing abiotic stress tolerance. Screening of transgenic T0 plants<br/>regenerated from both mature and immature embryos showed an average<br/>transformation % of about 4.612%. Screening of T1 mutants using the CAPS<br/>PCR method within 20 transformed plants showed successful mutagenesis for<br/>all lines selected, especially lines 2, 3, 8,12,13,14 which resulted in uncut<br/>bands. Sanger sequencing and alignment analysis of the mutated lines 2 and<br/>12 (MLs-2 and12) along with wild-type Giza 171 (WT-G171) ensured<br/>complete knockout in both lines. Stomatal measurements under an electron<br/>microscope revealed significant reductions in both stomatal dimensions.<br/>IV<br/>Drought tolerance evaluation of both lines under the effect of PEG showed the<br/>inability of WT-G171 to tolerate PEG concentrations >10% while MLs-2 and<br/>12 showed high significant improvement under 15 and 20% PEG. Salinity<br/>tolerance evaluation conducted on MLs-2, 3, 4, 8, 12, 13, 14, and 18 using 0,<br/>150, 200, and 300 mM of NaCl revealed different behavioral patterns of the<br/>lines either showed non-significant reductions or significant distinction when<br/>compared to themselves through all treatments thus considered as susceptible<br/>salinity tolerant genotypes (ex. ML-2 and ML-13) or showed significant<br/>reductions in the studied parameters (Eg. ML-4 and ML-18). Therefore, ML-<br/>2 can be considered as a susceptible abiotic stress-tolerant genotype and it is<br/>important to dive deeper into the study of the other susceptible tolerant<br/>mutated lines’ mutations, that gave it its excellency in most of the studied traits,<br/>both on the molecular level and field level. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
<br/>يمثل تغير المناخ العالمي تحديًا كبيرًا لإ نتاج الزراعي ، لا سيما في المناطق شبه الجافة والجافة ، حيث تتسبب ندرة<br/>المياه في خسائر فادحة في إنتاجية المحاصيل وذلك نتيجة للتغيرات المناخية العالمية المستمرة ،ومن المتوقع أن يؤثر انخفاض<br/>توافر المياه والتملح على ما يصل إلى50٪ من جميع الاراضى الصالحة للزراعة بحلول عام2050. يعتبر القمح أحد أكثر<br/>المحاصيل استهالكًا في جميع أنحاء العالم ، وخاصة في مصر ، و يمثل أكثر من20٪ من السعرات الحرارية اليومية. لذلك ،<br/>فإن تحسين تحمل القمح للجفاف وضغوط الملوحةالعالية(الاجهادات الغير حيوية) أمر ضرورى لضمان الامن الغذائي في<br/>الاونة الاخيرة ، تم استخدام نظامCRISPR / Cas9(Clustered Regularly Interspaced Short Palindromic<br/>Repeats/CRISPR associated nuclease 9) كأداة قوية لتحسين السمات بكفاءة عالية. لذلك ،تهدفهذه الدراسة إلى<br/>استخدام نظامCRISPR / Cas9لتثبيط عملجينSALI(مما أثر فى زيادة تحمل الجفاف في بعض النباتات بخلاف القمح) |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issues also as CD. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English and abstract in Arabic & English. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Wheat |
| General subdivision |
Genetics. |
| 653 #0 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Genome editing |
| -- |
CRISPR/Cas9 |
| -- |
Triticum aestivum |
| -- |
Abiotic stress |
| -- |
Biolistic delivery |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ezz Eldein Shafei, |
| Relator term |
thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mahmoud Saleh Mahmoud, |
| Relator term |
thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Nagwa Ibrahim Elarabi |
| Relator term |
thesis advisor. |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 900 ## - EQUIVALENCE OR CROSS-REFERENCE-PERSONAL NAME [LOCAL, CANADA] |
|---|
| Numeration |
15-08-2022 |
| Titles and other words associated with a name |
Ezz Eldein Shafei |
| -- |
Mahmoud Saleh Mahmoud |
| -- |
Nagwa Ibrahim Elarabi |
| Universities |
Cairo University |
| Faculties |
Faculty of Science |
| Divisons |
Department of chemistry |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Hanan |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
| Edition |
21 |
| Suppress in OPAC |
No |
