MARC details
| 000 -LEADER |
|---|
| fixed length control field |
05798namaa22004211i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20240211192450.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
231113s2023 ua a|||frm||| 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GICUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GICUC |
| Modifying agency |
EG-GICUC |
| Description conventions |
rda |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| Language code of summary or abstract |
eng |
| -- |
ara |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposit |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
636.089 |
| Edition number |
21 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) |
|---|
| Classification number |
636.089 |
| Edition number |
21 |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.10.10.M.Sc.2023.Ma.R |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Marwa Abdel Aal Reyad, |
| Relator term |
preparation. |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Restriction fragment length polymorphism genotyping of methicillin resistant staphylococcus aureus isolates from man and animals / |
| Statement of responsibility, etc. |
submitted by Marwa Abdel Aal Reyad ; under the supervision of Prof. Dr. Wagih A. Gad El-Said, Prof. Dr. Mahmoud D. El-Hariri, Prof. Dr. Sabry D. Morgan. |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
التصنيف الجيني لمعزولات الميكروب العنقودي المقاوم للمثيسلين من الانسان والحيوان بواسطة ار-اف-ال-بي (RFLP) |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Date of production, publication, distribution, manufacture, or copyright notice |
2023. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
72 pages : |
| Other physical details |
illustrations ; |
| Dimensions |
25 cm. + |
| Accompanying material |
CD. |
| 336 ## - CONTENT TYPE |
|---|
| Content type term |
text |
| Source |
rdacontent |
| 337 ## - MEDIA TYPE |
|---|
| Media type term |
Unmediated |
| Source |
rdamedia |
| 338 ## - CARRIER TYPE |
|---|
| Carrier type term |
volume |
| Source |
rdacarrier |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (M.Sc.)-Cairo University, 2023. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Bibliography: pages 56-72. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
A total of 101 pet dog samples (67nasal swabs and 34 wound swabs) were collected from 101 pet dogs. All samples were cultured for the isolation of Staphylococcus aureus using selective media and biochemical tests. Isolates were identified as MRSA after antimicrobial susceptibility testing. PCR for mecA gene was applied. Nineteen MRSA isolates were recovered from 67 (28%) nasal swabs and 6 wound isolates from 34 (17.6%). All isolates were resistant to cefoxitin and sensitive to both polymyxin B and vancomycin. All the 25 MRSA isolates were positive to mecA gene. A total of 53 pet cats 42 oropharyngeal swabs and 11 rectal swabs were collected. A total of 24 nasal swabs from human. All samples were cultured for the isolation of Staphylococcus aureus using selective media and biochemical tests. Isolates were identified as MRSA after antimicrobial susceptibility testing. PCR for mecA gene was applied. The investigation of MRSA in cats oropharyngeal and rectal swabs revealed (58.8 & 50%) respectively, for human was 21.4%. All isolates were resistant to cefoxitin and sensitive to both polymyxin B and vancomycin. Thirteen PCR amplicons from both cats & human were subsequently recognized by molecular analysis of spa gene polymorphism in clinical isolates by PCR-RFLP. The data were analyzed by GENEANALYZER software for actual analyzing and determining of amplicons size after HAEII restriction enzyme digestion of spa PCR product. A total of 8 different electrophoretic patterns were observed for the HaeII digested PCR products among the 13 strains of MRSA. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
تم جمع 101 عينة من كلاب أليفة (67 مسحة أنف و 34 مسحة جرح) من 101 كلب أليف. تمت زراعة جميع العينات لعزل المكورات العنقودية الذهبية باستخدام البيئة المتخصصة واختبارات كيميائية حيوية. تم التعرف على العزلات على أنها MRSA بعد اختبار الحساسية للمضادات الميكروبية. تم تطبيق PCR للجين mecA. تم الحصول على 19 عزلة أنفية من MRSA من 67 (28٪) مسحة أنف و6 عزلات جرح من 34 (17.6٪). كانت جميع العزلات مقاومة للسيفوكسيتين وحساسة لكل من البوليميكسين B والفانكومايسين. جميع عزلات MRSA البالغ عددها 25 كانت موجبة لجين mecA. تم جمع عينات من53 قطط أليف 42 مسحة فموي بلعومي و11 مسحة من المستقيم. تمت زراعة جميع العينات لعزل المكورات العنقودية الذهبية باستخدام وسط انتقائي واختبارات كيميائية حيوية. تم التعرف على العزلات على أنها MRSA بعد اختبار الحساسية للمضادات الميكروبية. تم تطبيق PCR للجين mecA. femA كشف فحص MRSA في مسحات البلعوم والفم في القطط (58.8 و 50٪) على التوالي .كما تم جمع 24 عينة من الانسان من خلال مسحات أنفية وكانت ايجابية MRSA بنسبة 21.4%. كانت جميع العزلات مقاومة للسيفوكسيتين وحساسة لكل من البوليميكسين B والفانكومايسين. تم التعرف على ثلاثة عشر منتج PCR لاحقًا من خلال التحليل الجزيئي لتعدد الأشكال الجيني في SPA في العزلات السريرية بواسطة PCR-RFLP. تم تحليل البيانات بواسطة برنامج GENEANALYZER للتحليل الفعلي وتحديد حجم الأمبليكون بعد هضم إنزيم القطع HAE II لمنتجSPA PCR. تم ملاحظة ما مجموعه 8 أنماط مختلفة للرحلان الكهربي لمنتجات Hae II المهضومة من بين 13 سلالة من MRSA |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issues also as CD. |
| 546 ## - LANGUAGE NOTE |
|---|
| Language note |
Text in English and abstract in Arabic & English. |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
Veterinary Medicine |
| Source of heading or term |
1 |
| 653 #0 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Staphylococcus aureus |
| -- |
MRSA |
| -- |
Pet cats |
| -- |
PCR |
| -- |
RFLP |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Wagih A. Gad El-Said, |
| Relator term |
thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mahmoud D. El-Hariri, |
| Relator term |
thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Sabry D. Morgan, |
| Relator term |
thesis advisor. |
| 900 ## - EQUIVALENCE OR CROSS-REFERENCE-PERSONAL NAME [LOCAL, CANADA] |
|---|
| Numeration |
01-01-2023 |
| Titles and other words associated with a name |
Wagih A. Gad El-Said |
| -- |
Mahmoud D. El-Hariri |
| -- |
Sabry D. Morgan |
| Dates associated with a name |
Ayman Tolba |
| -- |
Kariem Abdel Aziz |
| Universities |
Cairo University |
| Faculties |
Faculty of Veterinary Medicine |
| Divisons |
Department of Microbiology |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Asmaa |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
| Edition |
21 |
| Suppress in OPAC |
No |
