Next generation sequencing data analysis to unlock functional genomics of Dunaliella salina / (Record no. 168464)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 05616namaa22004451i 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - أخر تعامل مع التسجيلة | |
| control field | 20250223033315.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 241019s2023 |||a|||f m||| 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloguing agency | EG-GICUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GICUC |
| Modifying agency | EG-GICUC |
| Description conventions | rda |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | eng |
| -- | ara |
| 049 ## - Acquisition Source | |
| Acquisition Source | Deposite |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.62 |
| 092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) | |
| Classification number | 660.62 |
| Edition number | 21 |
| 097 ## - Degree | |
| Degree | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Local Call Number | Cai01.12.05.Ph.D.2023.He.N |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Authority record control number or standard number | Hend Yousuf Mohammad Yousuf, |
| Preparation | preparation. |
| 245 10 - TITLE STATEMENT | |
| Title | Next generation sequencing data analysis to unlock functional genomics of Dunaliella salina / |
| Statement of responsibility, etc. | by Hend Yousuf Mohammad Yousuf ; Supervisors: Prof. Dr.Zeinab Khalil Ibraheem , Prof. Dr.Fatma El-Zahraa Tawfeek , Dr.El-Adl Galal El-deen , Dr.Tamer Ahmed Mansour. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | / الجيل التالي من تحليل البيانات التسلسلية للكشف الوظيفي للجينوم في الدوناليلا سالينا |
| 264 #0 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | 2023. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 113 pages : |
| Other physical details | illustrations ; |
| Dimensions | 25 cm. + |
| Accompanying material | CD. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Source | rda content |
| 337 ## - MEDIA TYPE | |
| Media type term | Unmediated |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | volume |
| Source | rdacarrier |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D)-Cairo University, 2023. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Bibliography: pages 200-250. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Introduction: the growth of Dunaliella salina SA20 (Dunal) Teodoresco Egyptian <br/>isolate (from El-Bardawil Lake, Sinai, Egypt) in wastewater such as mozzarella cheese <br/>whey (MCW) is a more cost-effective cultivation method and growing D. salina SA20 <br/>in such waste could be used safely in biotechnological applications. <br/>Methodology: the use of 20% v/v MCW/Loeblich nutrient medium as growth <br/>supplement allowed mixotrophic nutrition, while growth in 100% Loeblich allowed for <br/>autotrophic nutrition. Mixotrophy and autotrophy were compared utilizing the next-<br/>generation sequencing technique, transcriptome assembly, and differential gene <br/>expression analysis using RNA-Seq. <br/>Results: the optimum growth performance of D. salina SA20 cells was observed in pH <br/>7.5 and was indicated by measured values of chlorophyll a (10.51 mg/L), dry weight <br/>(405 mg/L), cell count (6.92 x 106 cell/ml), and optical density (1.12). Additionally, a <br/>growth curve experiment comparing the mix- and autotrophic conditions presented <br/>higher values of the relative mean and maximum growth rates in mixotrophic <br/>conditions than in the autotrophic conditions. The pathway enrichment analysis under <br/>mixotrophic conditions found down-expression in important genes for nitrogen <br/>metabolism, carbon metabolism, purine and pyrimidine biosynthesis, and up-expression <br/>in genes encoding for enzymes involved in photosynthesis, carbon fixation, <br/>glucogenesis, and ribosome production pathways. <br/>Conclusion: the results might suggest a connection between the induced growth strategy <br/>used in mixotrophy and improved photosynthesis coupled with enhanced <br/>glucogenesis. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | تم استخدام شرش الموتزريلا والناتج بكميات كبيرة من صناعة الألبان كوسط غذائي لنموطحلب دوناليلا سالينا بطريقة آمنة في تطبيقات التكنولوجيا الحيوية وذلك بإضافته إلى الوسطالغذائي الغير عضوي )لوبلش( بتركيزات مختلفة) 20 و 50 و 75% شرش/لوبلش( كمثالللتغذية المختلطة بينما يعتبر النمو ف ي الوسط الغير عضوي )١٠٠% لوبلش( هو مثال للتغذيةالذاتية وقد دلت النتائج على ان التغذية المختلطة بتركيز ٢٠% قد سجلت أعلى النتائج )كلوروفيلأ 10.51 مجم/لتر والوزن الجاف 405 مجم/لتر وعدد الخلايا 6.92× 610 خلية/مل(، والكثافةالبصرية) 1.12(. كما تم استخدام قيم مختلفة للاس الهيدروجين ي لدراسة تأثير تلك القيم المختلفة حيث وجد انpH 7 هو القيمة المثلى للنمو. بالإضافة إلى ذلك، قدمت تجربة منحنى النمو التي تقارن بين ظروف التغذية المختلطة والتغذية الذاتية قيمًا أعلى للمتوسط النسبي ومعدلات النمو القصوى في ظروف التغذية المختلطة) 0.19, 0.21 /يوم، على التوالي( مقارنة بظروف التغذيةالذاتية) 0.16، 0.18/ يوم، على التوال ي(. كشف تحليل الترنسكربتوم عن إفراط في التعبير في الجينات المشاركة في التمثيل الضوئي، وتثبيت الكربون، وتكوين الجلوكوز، ومسارات تخليق الريبوسوم ،وتثبيط في مسارات النيتروجين، وأيض الكربون، والتخليق الحيوي للبيورينوالبيريميدين. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Issues CD | Issues also as CD. |
| 546 ## - LANGUAGE NOTE | |
| Text Language | Text in English and abstract in Arabic & English. |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Microbiology |
| Source of heading or term | qrmak |
| 653 #0 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | differential gene expression analysis |
| -- | Dunaliella salina |
| -- | growth supplements |
| -- | mixotrophy |
| -- | whey |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zeinab Khalil Ibraheem |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Fatma El-Zahraa Tawfeek |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | El-Adl Galal El-deen |
| Relator term | thesis advisor. |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tamer Ahmed Mansour |
| Relator term | thesis advisor. |
| 900 ## - Thesis Information | |
| Grant date | 01-01-2023 |
| Supervisory body | Zeinab Khalil Ibraheem |
| -- | Fatma El-Zahraa Tawfeek |
| -- | El-Adl Galal El-deen |
| -- | Tamer Ahmed Mansour |
| Universities | Cairo University |
| Faculties | Faculty of Science |
| Department | Department of Botany and Microbiology |
| 905 ## - Cataloger and Reviser Names | |
| Cataloger Name | Shimaa |
| Reviser Names | Huda |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Edition | 21 |
| Suppress in OPAC | No |
| Source of classification or shelving scheme | Home library | Current library | Date acquired | Inventory number | Full call number | Barcode | Date last seen | Effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 19.10.2024 | 88781 | Cai01.12.05.Ph.D.2023.He.N | 01010110088781000 | 19.10.2024 | 19.10.2024 | Thesis |