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Advanced techniques for the detection and differentiation between whitefly - transmitted begeminiviruses / (Record no. 3214)

MARC details
000 -LEADER
fixed length control field 025850000a22003130004500
003 - CONTROL NUMBER IDENTIFIER
control field EG-GICUC
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 060930s2006 ua a f m 000 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EG-GICUC
Language of cataloging eng
Transcribing agency EG-GICUC
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title Eng
049 ## - LOCAL HOLDINGS (OCLC)
Holding library Deposite
097 ## - Thesis Degree
Thesis Level Ph.D
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number Cai01.07.16.Ph.D.2006.Do.A.
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Doaa Zeinhom Ramadan Soliman
245 10 - TITLE STATEMENT
Title Advanced techniques for the detection and differentiation between whitefly - transmitted begeminiviruses /
Statement of responsibility, etc. Doaa Zeinhom Ramadan Soliman ; supervised Aly Maamoun Abd El Salam , Naglaa Abd El Monem Abdallah
246 15 - VARYING FORM OF TITLE
Title proper/short title تقنيات متقدمة للكشف والتفرقة بين الفيروسات التوامية المنقولة بالذبابة البيضاء
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Cairo :
Name of publisher, distributor, etc. Doaa Zeinhom Ramadan Soliman ,
Date of publication, distribution, etc. 2006
300 ## - PHYSICAL DESCRIPTION
Extent 113P :
Other physical details ill ;
Dimensions 25cm
502 ## - DISSERTATION NOTE
Dissertation note Thesis (PH.D.) - Cairo University - Faculty Of Agriculture - Department Of Plant Pathology and Physiology
520 ## - SUMMARY, ETC.
Summary, etc. Total DNA for ten begomoviruses were successfully extracted from tissues of healthy and infected plant grown in field and greenhouse by applying CTAB methodThe PCR was used in the detection of begomoviruses under study in tissues of infected plants growing in the fieldThe degenerate primers (AV - core and AC - core) for begomoviruses were able to amplify products of 576 - 579 bp of core coat protein of all viruses under study except for CLCMVAlso results indicated the efficiency of IC - PCR in the detection of OLCV and TYLCV in sap extracted from infected plants growing in the field using 1 / 500 TYLCV - CP antiserumNon - radioactive hybridization technique was employed in order to detect and differentiate between begomoviruses under study in both of infected and uninfected plant tissuesThe non radioactive - labeled probe of SLCV was prepared using PCR amplification with degenerate primers of core coat protein of geminivirusesSLCV labeled probe was used in squash , dot , and Southern blot hybridization techniquesResults indicated a close sequence homology between SLCV and TYLCV , and a moderate sequence homology with each of OLCV , HLCrV and OYVVIn addition , results predicted that DNA sequences of the other viruses and SLCV are less conserved
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE
Additional physical form available note Issued also as CD
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Advanced Techniques
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Detection and Differentiation
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Whitefly - Transmitted Geminiviruses
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Aly Maamoun Abd El Salam ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Naglaa Abd El Monem Abdallah ,
Relator term
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Enas
Reviser Cataloger
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Mustafa
Reviser Revisor
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Thesis
Holdings
Source of classification or shelving scheme Not for loan Home library Current library Date acquired Full call number Barcode Date last seen Koha item type
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة قاعة الرسائل الجامعية - الدور الاول 11.02.2024 Cai01.07.16.Ph.D.2006.Do.A. 01010110045446000 22.09.2023 Thesis
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة مخـــزن الرســائل الجـــامعية - البدروم 11.02.2024 Cai01.07.16.Ph.D.2006.Do.A. 01020110045446000 22.09.2023 CD - Rom