MARC details
000 -LEADER |
fixed length control field |
025850000a22003130004500 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
EG-GICUC |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
060930s2006 ua a f m 000 0 eng d |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
EG-GICUC |
Language of cataloging |
eng |
Transcribing agency |
EG-GICUC |
041 0# - LANGUAGE CODE |
Language code of text/sound track or separate title |
Eng |
049 ## - LOCAL HOLDINGS (OCLC) |
Holding library |
Deposite |
097 ## - Thesis Degree |
Thesis Level |
Ph.D |
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
Classification number |
Cai01.07.16.Ph.D.2006.Do.A. |
100 0# - MAIN ENTRY--PERSONAL NAME |
Personal name |
Doaa Zeinhom Ramadan Soliman |
245 10 - TITLE STATEMENT |
Title |
Advanced techniques for the detection and differentiation between whitefly - transmitted begeminiviruses / |
Statement of responsibility, etc. |
Doaa Zeinhom Ramadan Soliman ; supervised Aly Maamoun Abd El Salam , Naglaa Abd El Monem Abdallah |
246 15 - VARYING FORM OF TITLE |
Title proper/short title |
تقنيات متقدمة للكشف والتفرقة بين الفيروسات التوامية المنقولة بالذبابة البيضاء |
260 ## - PUBLICATION, DISTRIBUTION, ETC. |
Place of publication, distribution, etc. |
Cairo : |
Name of publisher, distributor, etc. |
Doaa Zeinhom Ramadan Soliman , |
Date of publication, distribution, etc. |
2006 |
300 ## - PHYSICAL DESCRIPTION |
Extent |
113P : |
Other physical details |
ill ; |
Dimensions |
25cm |
502 ## - DISSERTATION NOTE |
Dissertation note |
Thesis (PH.D.) - Cairo University - Faculty Of Agriculture - Department Of Plant Pathology and Physiology |
520 ## - SUMMARY, ETC. |
Summary, etc. |
Total DNA for ten begomoviruses were successfully extracted from tissues of healthy and infected plant grown in field and greenhouse by applying CTAB methodThe PCR was used in the detection of begomoviruses under study in tissues of infected plants growing in the fieldThe degenerate primers (AV - core and AC - core) for begomoviruses were able to amplify products of 576 - 579 bp of core coat protein of all viruses under study except for CLCMVAlso results indicated the efficiency of IC - PCR in the detection of OLCV and TYLCV in sap extracted from infected plants growing in the field using 1 / 500 TYLCV - CP antiserumNon - radioactive hybridization technique was employed in order to detect and differentiate between begomoviruses under study in both of infected and uninfected plant tissuesThe non radioactive - labeled probe of SLCV was prepared using PCR amplification with degenerate primers of core coat protein of geminivirusesSLCV labeled probe was used in squash , dot , and Southern blot hybridization techniquesResults indicated a close sequence homology between SLCV and TYLCV , and a moderate sequence homology with each of OLCV , HLCrV and OYVVIn addition , results predicted that DNA sequences of the other viruses and SLCV are less conserved |
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
Additional physical form available note |
Issued also as CD |
653 #4 - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Advanced Techniques |
653 #4 - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Detection and Differentiation |
653 #4 - INDEX TERM--UNCONTROLLED |
Uncontrolled term |
Whitefly - Transmitted Geminiviruses |
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Aly Maamoun Abd El Salam , |
Relator term |
|
700 0# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Naglaa Abd El Monem Abdallah , |
Relator term |
|
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
Cataloger |
Enas |
Reviser |
Cataloger |
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
Cataloger |
Mustafa |
Reviser |
Revisor |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
Dewey Decimal Classification |
Koha item type |
Thesis |