MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03022cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
141118s2014 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.11.13.Ph.D.2014.Ha.M |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Hanaa Mohamed Mohamed Elzahed |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Molecular identification of blood stains on different surfaces and fabrics using specific multiplex mRNA / |
| Statement of responsibility, etc. |
Hanaa Mohamed Mohamed Elzahed ; Supervised Osama Mohamed Ebrahim Albarany , Amany Fahmy Hanon , Dina Sabry Abdelfatah |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
الاستعراف الجزيئي لبقع الدم علي الأسطح و الأنسجة المختلفة باستخدام التفاعل المتعدد المحدد للحمض النووي الريبوزى الراسل |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Hanaa Mohamed Mohamed Elzahed , |
| Date of publication, distribution, etc. |
2014 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
123 P. : |
| Other physical details |
charts , facsimiles ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Forensic Medicine and Clinical Toxicology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
In current forensic practice, blood stain identification is determined using enzymatic, immunologic and chemical testing. Recently, mRNA-profiling has emerged as an alternative strategy to identify the blood stains. mRNA profile clearly reveals tissue-specific gene expression patterns. HBB and HBA (the alpha and beta subunits of hemoglobin A) are specific mRNA blood biomarkers. This study aimed at identification of blood stains on different tested surfaces and fabrics for forensic application by molecular assessment of specific mRNA blood biomarkers. For this study, 100 blood samples were collected from healthy volunteers and they were classified into: (1) 9 groups, each group included 10 samples that were applied on 9 different fabrics and surfaces; cotton, wool, silk, synthetic nylon, wood, glass, ceramic, iron as a metal and leather. (2) One blood group included 10 samples that were applied on glass and exposed to humidity and high temperature to test their effect on mRNA. Ten saliva samples were collected from healthy volunteers and were applied on glass as a negative control body fluid. All samples were left to dry for 1 day. The next steps of the study were RNA extraction from blood and saliva dried stains, reverse transcription, performing gene expression analysis of the blood specific markers using RT-PCR then detection of the amplified gene using agarose gel electrophoresis. The results demonstrated that HBB and HBA can be recovered from blood stains in sufficient quantity and quality for mRNA analysis |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Blood stains |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Body fluid identification |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
globin genes |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Amany Fahmy Hanon , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Dina SabryAbdelfatah , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Osama Mohamed Ebrahim Albarany , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Aml |
| Reviser |
Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
