Differentiation between mycobacterium bovis and mycobacterium tuberculosis in cattle milk and lymphnodes using multiplex / (Record no. 51906)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 02586cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031256.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 150810s2014 ua h f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.10.M.Sc.2014.Su.D |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Susan Abdelnafea Ismail Mohamed |
| 245 10 - TITLE STATEMENT | |
| Title | Differentiation between mycobacterium bovis and mycobacterium tuberculosis in cattle milk and lymphnodes using multiplex / |
| Statement of responsibility, etc. | Susan Abdelnafea Ismail Mohamed ; Supervised Khaled Farouk Elamry , Salah Eldin Abdelkarim Selim , Mohamed Galal Aggour |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | التمييز بين مايكوباكتيريم بوفيس و مايكوباكتيريم تيوبركيلوزيس فى الالبان و الغدد الليمفاوية للماشية باستخدام تفاعل البلمرة التسلسى اللحظى المتعدد |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Susan Abdelnafea Ismail Mohamed , |
| Date of publication, distribution, etc. | 2014 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 109 P. : |
| Other physical details | facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Mycobacterium bovis, a member of the M. tuberculosis complex (MTC), is the causative agent of bovine tuberculosis. Detection of M. bovis and M. tuberculosis using conventional culture - and biochemical - based assays is time - consuming. Therefore, a simple and sensitive molecular assay for rapid detection would be of great help in specific situations such as faster diagnosis of bovine tuberculosis (bTB) infection in the abattoirs. A novel multiplex real-time PCR assay was developed and applied directly to biological samples with evidence of bTB and it was allowed to differentiate between M. bovis and M. tuberculosis. The primers and TaqMan probes were designed to target the IS1081gene, the multi-copy insertion element in the MTC and the 12.7 - kb fragment which presents in M. tuberculosis, not in the M. bovis genome. The assay was optimized and validated by testing 10 species of mycobacteria including M. bovis and M. tuberculosis, and 4 other bacterial species such as escherichia coli |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | M. tuberculosis complex |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Mycobacterium bovis |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Real time PCR |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Khaled Farouk Elamry , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Galal Aggour , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Salah Eldin Abdelkarim Selim , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.M.Sc.2014.Su.D | 01010110066179000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.M.Sc.2014.Su.D | 01020110066179000 | 22.09.2023 | CD - Rom | 66179.CD |