In vitro studies on drought tolerance using gene transfer on tomato / (Record no. 53123)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02438cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031330.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 151105s2015 ua h f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.05.M.Sc.2015.Do.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Doaa Hassan Ali Soliman |
| 245 10 - TITLE STATEMENT | |
| Title | In vitro studies on drought tolerance using gene transfer on tomato / |
| Statement of responsibility, etc. | Doaa Hassan Ali Soliman ; Supervised Ahmed Hussein Hanafy Ahmed , Ayman Yehia Amin , Hamdy Abdeaziz Moursy |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات معملية على تحمل الجفاف في الطماطم باستخدام النقل الجيني |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Doaa Hassan Ali Soliman , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 99 P. : |
| Other physical details | facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Agriculture Botany |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The aim of this study is to investigate best conditions of previously published protocols on Agrobacterium-mediated transformation of tomato, to deliver PtdIns PLC2 gene, which renders the trait of tolerance to drought, to Moneymaker (MM), Super Strain B (SSB) and Rio Grande (RG) tomato cultivars. The obtained results indicated that the efficiency of Agrobacterium-mediated gene transfer in tomato tissues is influenced by many factors; the most important are cultivars, explants type and bacterial optical density. The highest transformation efficiency was 68% with Moneymaker flamingo bill like explant. Whereas the transformation efficiency with Super Strain B and Rio Grande cotyledons were 48% and 40%, respectively. On contrary, the transformation efficiency of Rio Grande and Super Strain B hypocotyls were 20% and 16 %, respectively. The best optical density for cotyledons and hypocotyls of the SSB and RG was 0.05 followed by 0.5 and 1.0. The presence of transgenes and their expression were confirmed by molecular analysis (PCR, RT PCR and Real Time PCR) and histochemical analysis (GUS assay) |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Agrobacterium tumefaciensis |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | PtdIns PLC2 |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Tomatoes |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ahmed Hussein Hanafy Ahmed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ayman Yehia Amin , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hamdy Abdeaziz Moursy , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Aml |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.05.M.Sc.2015.Do.I | 01010110066592000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.05.M.Sc.2015.Do.I | 01020110066592000 | 22.09.2023 | CD - Rom | 66592.CD |