First detection of deformed wing virus (DWV) and Kakugo virus (KV) in honeybees (Apis mellifera L.) (Hymenoptera - Apidae) in Egypt by RT - PCR / (Record no. 53423)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02220cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 151121s2015 ua h f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.12.14.M.Sc.2015.He.F |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Heba Allah Elsayed Ibrahim Mohammed Seyam |
| 245 10 - TITLE STATEMENT | |
| Title | First detection of deformed wing virus (DWV) and Kakugo virus (KV) in honeybees (Apis mellifera L.) (Hymenoptera - Apidae) in Egypt by RT - PCR / |
| Statement of responsibility, etc. | Heba Allah Elsayed Ibrahim Mohammed Seyam ; Supervised Fatma Kamel Adham , Emtithal Mohammed Abdelsamiea , Sawsan Elmohandes |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | RT - PCR باستخدام DWV , KV الكشف لأول مرة فى مصر لنوعين من فيروسات النحل |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Heba Allah Elsayed Ibrahim Mohammed Seyam , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 128 P. : |
| Other physical details | facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Entomology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Total RNA was isolated for deformed wing virus (DWV) and Kakugo virus (KV) from honeybees. Viral detection and identification was confirmed by sequencing of the PCR products using conventional RT - PCR. Homology search of DWV PCR amplified fragment revealed similarities of 99% with DWV isolate Warwick - 2009 poly - protein gene (accession number: GU109335.1) and 98% with DWV isolate Chilensis A complete genome (accession number: JQ413340.1). Also, homology search of KV PCR amplified fragment revealed similarities of 97% with KV isolate - Ox genomic sequence (accession number: KC786224.1), 90.9% with DWV - Ox genomic sequence (accession number: KC786223.1) The described real - time RT - PCR SG RT - PCR proved to be a fast, accurate and useful technique to detect and quantify low viral loads in honeybees and varroa mites |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Deformed wing virus |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Kakugo virus |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Varroa mites and RT - PCR |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Emtithal Mohammed Abdelsamiea , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Fatma Kamel Adham , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sawsan Elmohandes , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.12.14.M.Sc.2015.He.F | 01010110066800000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.12.14.M.Sc.2015.He.F | 01020110066800000 | 22.09.2023 | CD - Rom | 66800.CD |