MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02860cam a2200313 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
151213s2015 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.11.07.Ph.D.2015.Di.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Dina Mohamed Tawfik Koptan |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Ex vivo expansion of oral mucosal epithelial stem cells on amniotic membrane for potential use in ocular surface reconstruction / |
| Statement of responsibility, etc. |
Dina Mohamed Tawfik Koptan ; Supervised Mervat Mohamed Saad Elansary , Nancy Mohamed Elguindy |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
دراسة معملية لزراعة الخلايا الجذعية من الغشاء المخاطى المبطن للفم على الغشاء الأمنيونى لإمكانية إعادة إعمار سطح العين |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Dina Mohamed Tawfik Koptan , |
| Date of publication, distribution, etc. |
2015 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
115 P. : |
| Other physical details |
charts , facsimiles ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Background: Stem cells of the corneal epithelium are located at the limbus. They are ultimately responsible for renewal and regeneration of the corneal epithelium under normal circumstances and during wound healing. Diseases causing either complete loss of limbal stem cells or severe destruction of the limbal stroma result in the pathologic state of limbal stem cell deficiency. A new technique termed cultivated oral mucosal epithelial transplantation (COMET) has been developed to treat patients with limbal stem cell deficiency. Methods: Oral biopsies were obtained from three potential COMET candidates after their informed consent and were cultured as explants on denuded freeze-dried amniotic membrane for 24 days. Cultures were examined by light microscopy and immunohistochemistry. Results: Sheets of healthy, stratified oral epithelial cells were obtained at day 24. Cultured oral epithelial cells expressed marker of epithelial differentiation keratin 3. The cells also expressed p63 (a marker of poorly differentiated epithelial cells). The cells did not express cornea-specific keratin 12. Conclusion: Oral epithelial cells can be cultured as explants on denuded freeze dried amniotic membrane without using feeder cells. Characterization showed that these cells maintain the phenotypic characteristics of oral epithelial cells and that the culture is a heterogeneous population of differentiated and poorly differentiated cells |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Limbal stem cell deficiency |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Ocular surface reconstruction |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Oral mucosal epithelium |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mervat Mohamed Saad ElAnsary , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Nancy Mohamed ElGuindy , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Enas |
| Reviser |
Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
