MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02831cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
160224s2015 ua dhb f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.11.26.Ph.D.2015.Im.M |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Iman Raafat Mohamed Riad |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Molecular detection of wuchereria bancrofti in human blood and mosquitoes from selected endemic areas in Egypt / |
| Statement of responsibility, etc. |
Iman Raafat Mohamed Riad ; Supervised Ayman Abdelmoamen Elbadry , Eman Yassien Shoeib , Samar Sayed Attia |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
الكشف الجزيئي عن الفخرية البنكروفتية في دم الإنسان و في البعوض من مناطق موبوءة مختارة في مصر |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Iman Raafat Mohamed Riad , |
| Date of publication, distribution, etc. |
2015 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
158 P. : |
| Other physical details |
charts , facsimiles , maps ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Parasitology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Lymphatic filariasis is a vector-borne health problem, which have a profound impact on patient{u2019}s lives and can lead to permanent disability. Wuchereria bancrofti (W. bancrofti) is the major cause of filariasis worldwide and is focally endemic in Egypt. Diagnosis of filarial infection using traditional morphologic and immunological criteria can be challenging and lead to misdiagnosis. The aim of the present cross-sectional study was the molecular detection of W. bancrofti in human blood and mosquitoes from selected endemic areas in Egypt. Blood samples were collected from 300 individuals residing in filariasis endemic areas, and were subjected to ELISA for the detection of W. bancrofti antigens and semi-nested PCR targeting W. bancrofti repeated DNA sequences. Mosquito pools from same endemic areas were collected, sorted, and subjected to semi-nested PCR. Additionally, a group of positive PCR products were subjected to DNA sequencing and phylogenetic analysis. Results revealed that out of the 300 collected blood samples; 7 samples were positive by ELISA (2.3%), 45 samples were positive by semi-nested PCR (15%) and 3 samples were positive by both tests (1%). All the collected mosquito pools were negative. Sequences analysis confirmed semi-nested PCR results; identifying only W. bancrofti species. Sequence alignment and phylogenetic analysis indicated genetically distinct clusters of W. bancrofti among the study population |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
phylogenetic analysis |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Semi-nested PCR |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Wuchereria bancrofti |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Ayman Abdelmoamen Elbadry , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Eman Yassien Shoeib , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Samar Sayed Attia , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Soheir |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
