MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02871cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
160416s2015 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.08.08.Ph.D.2015.Ba.P |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Bahgat Mohammed Ezzat Bahgat Fayed |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Production and formulation of novel antibiotics from streptomyces / |
| Statement of responsibility, etc. |
Bahgat Mohammed Ezzat Bahgat Fayed ; Supervised Omaima Naim Elgazayerly , Magdy Ali Amin , Amal Mohamed Abdelgawad Hashem |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
إنتاج و صياغة مضادات حيوية جديدة منتجة بطرق مبتكرة من بكتريا الأستربتوميسيس |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Bahgat Mohammed Ezzat Bahgat Fayed , |
| Date of publication, distribution, etc. |
2015 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
208 P. : |
| Other physical details |
charts , facsimiles ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Pharmaceutics |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
Here we explore the use of multiple orthologous integrating plasmid systems, based on the int/attP loci from phages TG1, SV1 and zBT1, to express the PKS for erythromycin in a heterologous Streptomyces host. Streptomyces strains containing the three polyketide synthase genes, eryAI, eryAII and eryAIII expressed from three different integrated plasmids produced the aglycone intermediate, 6-deoxyerythronolide B. A further pair of integrating plasmids, both derived from the zC31 int/attP locus, were constructed encoding a gene cassette for glycosylation of the aglycone intermediates, with and without the tailoring gene, eryF, required for the synthesis of erythronolide B. Liquid chromatography {u2013} mass spectrometry of the metabolites indicated the production of angolosaminyl-6dEB and angolosaminyl-EB. Erythromycin was formulated into PLGA nanoparticles. Entrapment efficiency of erythromycin in PLGA nanoparticles was determined microbiologically. The effect of PLGA amount, PLGA type, organic solvent, sonication probe diameter, sonication time, organic phase: aqueous phase ratio as well as effect of PVA percent on microbiologically determined entrapment efficiency was assessed. The results showed that microbiologically determined entrapment efficiency was significantly enhanced after optimizing different parameters to reach 51.1±0.68 % for the optimum formula (F10). The size of the nanoparticles produced by optimal formulation observed from SEM micrographs was around 100 nm with narrow particle size distribution |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
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| Uncontrolled term |
Erythromycin |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Integrating plasmids |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Nanoparticles |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Amal Mohamed Abdelgawad Hashem , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Magdy Ali Amin , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Omaima Naim Elgazayerly , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Soheir |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
