Cloning of human granulocyte colony stimulating factor in prokaryotic cells / (Record no. 56065)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02258cam a2200313 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160418s2015 ua h f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.12.05.M.Sc.2015.Mo.C |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Mahmoud Mohamed Shaheen |
| 245 10 - TITLE STATEMENT | |
| Title | Cloning of human granulocyte colony stimulating factor in prokaryotic cells / |
| Statement of responsibility, etc. | Mohamed Mahamoud Mohamed Shaheen ; Supervised Mohamed Hani Moubasher , Mohamed Ali Saber |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | استنساخ معامل حث المستعمرة الحبيبية البشرى داخل خلايا أوليات النواة |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Mohmed Mahamoud Mohamed Shaheen , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 109 P. : |
| Other physical details | facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany and Microbiology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Human Granulocyte colony-stimulating factor (hG-CSF) is a growth factor or cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. In our study we aimed to optimize production (expression) of recombinant human G-CSF protein by two different strategies. The first one is based on the isolation of natural human G-CSF mRNA from polymorphonuclear cells (PMNCs). The second one is based on the synthesis of hG-CSF gene based on the GenBank published sequence. The hG-CSF open reading frame (ORF) of both strategies were amplified and cloned into pET-3a expression vector and transformed into BL21 DE3 E. coli cells for expression of recombinant hG-CSF protein and was successfully expressed and tested by Western blot. The results of this work indicate that successful expression of recombinant hG-CSF protein by two strategies which confirmed by the molecular weight and the immunogenicity against the hG-CSF antibodies |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Granulocyte colony-stimulating factor (G-CSF) |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Growth factor, |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | pET-3a expression system |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Ali Saber , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Hani Moubasher , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Soheir |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.12.05.M.Sc.2015.Mo.C | 01010110068553000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.12.05.M.Sc.2015.Mo.C | 01020110068553000 | 22.09.2023 | CD - Rom | 68553.CD |