Laboratory diagnosis of campylobacteriosis / (Record no. 56605)
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000 -LEADER | |
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fixed length control field | 02886cam a2200313 a 4500 |
003 - CONTROL NUMBER IDENTIFIER | |
control field | EG-GiCUC |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
fixed length control field | 160523s2015 ua o f m 000 0 eng d |
040 ## - CATALOGING SOURCE | |
Original cataloging agency | EG-GiCUC |
Language of cataloging | eng |
Transcribing agency | EG-GiCUC |
041 0# - LANGUAGE CODE | |
Language code of text/sound track or separate title | eng |
049 ## - LOCAL HOLDINGS (OCLC) | |
Holding library | Deposite |
097 ## - Thesis Degree | |
Thesis Level | M.Sc |
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
Classification number | Cai01.10.10.M.Sc.2015.Am.L |
100 0# - MAIN ENTRY--PERSONAL NAME | |
Personal name | Amal Mohammed Fouad Mohamed |
245 10 - TITLE STATEMENT | |
Title | Laboratory diagnosis of campylobacteriosis / |
Statement of responsibility, etc. | Amal Mohammed Fouad ; Supervised Jakeen Kamal Abdelhaleem Eljakee , Soad Abdelaziz Abdelwanis |
246 15 - VARYING FORM OF TITLE | |
Title proper/short title | التشخيص المعملى لمرض الكامبيلوباكتر |
260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
Place of publication, distribution, etc. | Cairo : |
Name of publisher, distributor, etc. | Amal Mohammed Fouad Mohamed , |
Date of publication, distribution, etc. | 2015 |
300 ## - PHYSICAL DESCRIPTION | |
Extent | 174 P. : |
Other physical details | photographs ; |
Dimensions | 25cm |
502 ## - DISSERTATION NOTE | |
Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology |
520 ## - SUMMARY, ETC. | |
Summary, etc. | Out of 150 samples (50 from imported chicks,ducklings), (50 from imported frozen chicken carcasses) and (50 from native poultry) the incidence of campylobacter was 11.33%. Campylobacter was isolated from fecal matter of imported chicks,drip of frozen chicken carcasses and cloacal swabs of native poultry and it was found that the highest percent of isolation was from native poultry16% followed by imported birds 14%,while the lowest isolation rate was from frozen chicken carcasses 4%. By cultivationof 6 isolates of Campylobacter by three different media (mCCDA, karmali, preston), it was found that mCCDA medium was the most effective medium for enumeration of campylobacter followed by karmali then preston. An inter laboratory experiment design was held to compare several techniques for detection of lowest number of campylobacter cells in chicken meat sample,it was found that enrichment in bolton broth followed by plating on mCCDA and PCR were able to detect as low as 10 cfu/gm of sample. Detection of 5 of genes (flaA) and (cdtA) and (virB11) and (iam) and (wlan) associated with virulence factors were done by PCR, and the study showed the the most prevalent gene was flaA gene70.6% followed by cdtA gene 47% then wlan 17.6% and iam 11.8%, while no virB gene could be found in this study. Also,in this study comparison of (i) cryobank microbial preservation system using(i) hypertonic {u2018}cryopreservative solution{u2019} and glass beads, (ii) FBP medium, (iii) 20% glycerol / 80% nutrient broth culture, and (iv) 50% glycerol / 50% nutrient broth (v) bovine and swine serums,and Bovine serum preservation technique proved the most successful preservation technique with best viability of the recovered cells for 160 days |
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
Additional physical form available note | Issued also as CD |
653 #4 - INDEX TERM--UNCONTROLLED | |
Uncontrolled term | Campylobacter |
653 #4 - INDEX TERM--UNCONTROLLED | |
Uncontrolled term | Isolation enumeration |
653 #4 - INDEX TERM--UNCONTROLLED | |
Uncontrolled term | Virulence factors |
700 0# - ADDED ENTRY--PERSONAL NAME | |
Personal name | Jakeen Kamal Abdelhaleem Eljakee , |
Relator term | |
700 0# - ADDED ENTRY--PERSONAL NAME | |
Personal name | Soad Abdelaziz Abdelwanis , |
Relator term | |
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
Cataloger | Nazla |
Reviser | Revisor |
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
Cataloger | Samia |
Reviser | Cataloger |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Source of classification or shelving scheme | Dewey Decimal Classification |
Koha item type | Thesis |
Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
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Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.10.M.Sc.2015.Am.L | 01010110068973000 | 22.09.2023 | Thesis | ||
Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.10.M.Sc.2015.Am.L | 01020110068973000 | 22.09.2023 | CD - Rom | 68973.CD |