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Laboratory diagnosis of campylobacteriosis / (Record no. 56605)

MARC details
000 -LEADER
fixed length control field 02886cam a2200313 a 4500
003 - CONTROL NUMBER IDENTIFIER
control field EG-GiCUC
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 160523s2015 ua o f m 000 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EG-GiCUC
Language of cataloging eng
Transcribing agency EG-GiCUC
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
049 ## - LOCAL HOLDINGS (OCLC)
Holding library Deposite
097 ## - Thesis Degree
Thesis Level M.Sc
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number Cai01.10.10.M.Sc.2015.Am.L
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Amal Mohammed Fouad Mohamed
245 10 - TITLE STATEMENT
Title Laboratory diagnosis of campylobacteriosis /
Statement of responsibility, etc. Amal Mohammed Fouad ; Supervised Jakeen Kamal Abdelhaleem Eljakee , Soad Abdelaziz Abdelwanis
246 15 - VARYING FORM OF TITLE
Title proper/short title التشخيص المعملى لمرض الكامبيلوباكتر
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Cairo :
Name of publisher, distributor, etc. Amal Mohammed Fouad Mohamed ,
Date of publication, distribution, etc. 2015
300 ## - PHYSICAL DESCRIPTION
Extent 174 P. :
Other physical details photographs ;
Dimensions 25cm
502 ## - DISSERTATION NOTE
Dissertation note Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520 ## - SUMMARY, ETC.
Summary, etc. Out of 150 samples (50 from imported chicks,ducklings), (50 from imported frozen chicken carcasses) and (50 from native poultry) the incidence of campylobacter was 11.33%. Campylobacter was isolated from fecal matter of imported chicks,drip of frozen chicken carcasses and cloacal swabs of native poultry and it was found that the highest percent of isolation was from native poultry16% followed by imported birds 14%,while the lowest isolation rate was from frozen chicken carcasses 4%. By cultivationof 6 isolates of Campylobacter by three different media (mCCDA, karmali, preston), it was found that mCCDA medium was the most effective medium for enumeration of campylobacter followed by karmali then preston. An inter laboratory experiment design was held to compare several techniques for detection of lowest number of campylobacter cells in chicken meat sample,it was found that enrichment in bolton broth followed by plating on mCCDA and PCR were able to detect as low as 10 cfu/gm of sample. Detection of 5 of genes (flaA) and (cdtA) and (virB11) and (iam) and (wlan) associated with virulence factors were done by PCR, and the study showed the the most prevalent gene was flaA gene70.6% followed by cdtA gene 47% then wlan 17.6% and iam 11.8%, while no virB gene could be found in this study. Also,in this study comparison of (i) cryobank microbial preservation system using(i) hypertonic {u2018}cryopreservative solution{u2019} and glass beads, (ii) FBP medium, (iii) 20% glycerol / 80% nutrient broth culture, and (iv) 50% glycerol / 50% nutrient broth (v) bovine and swine serums,and Bovine serum preservation technique proved the most successful preservation technique with best viability of the recovered cells for 160 days
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE
Additional physical form available note Issued also as CD
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Campylobacter
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Isolation enumeration
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Virulence factors
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Jakeen Kamal Abdelhaleem Eljakee ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Soad Abdelaziz Abdelwanis ,
Relator term
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Nazla
Reviser Revisor
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Samia
Reviser Cataloger
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Thesis
Holdings
Source of classification or shelving scheme Not for loan Home library Current library Date acquired Full call number Barcode Date last seen Koha item type Copy number
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة قاعة الرسائل الجامعية - الدور الاول 11.02.2024 Cai01.10.10.M.Sc.2015.Am.L 01010110068973000 22.09.2023 Thesis  
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة مخـــزن الرســائل الجـــامعية - البدروم 11.02.2024 Cai01.10.10.M.Sc.2015.Am.L 01020110068973000 22.09.2023 CD - Rom 68973.CD