Some trials for cryobanking of Egyptian buffalo oocytes / (Record no. 56687)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02979cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160529s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.14.M.Sc.2016.Am.S |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Amr Salah Mohamed Elshalofy |
| 245 10 - TITLE STATEMENT | |
| Title | Some trials for cryobanking of Egyptian buffalo oocytes / |
| Statement of responsibility, etc. | Amr Salah Mohamed Elshalofy ; Supervised Aly B. A. Badawy , Sayed T. Ismail , Gamal M. Darwish |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | بعض المحاولات لحفظ بويضات الجاموس المصرى عن طريق التجميد |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Amr Salah Mohamed Elshalofy , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 120 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Theriogenology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | A total number of 2074 cumulus oocyte complexes (COCs) were aspirated from 1488 ovary collected from a local slaughterhouse (Cairo, Egypt) from January 2014 to October 2015, then COCs, with at least two to three compact layers of cumulus cells and a homogeneous cytoplasm, were selected under a stereomicroscope for the following experiments. Experiment 1, studied the effect of different CPAs and CPAs combinations on maturation of (GV) stage buffalo oocytes in vitro. The maturation rates of (GV) buffalo oocytes were accounted to be (38.10%, 32.00%, 26.51%, 24.07%and 22.00%) after exposure to EG40%, EG20% + GLY20%, EG20% + DMSO20%, EG20% and EG10% CPAs, respectively. Experiment 2, the removal of sucrose from these CPAs solutions has resulted in a significant (P < 0.05) increase in this criteria (59.74%, 51.85% and 40.26%) after exposure to EG20% + DMSO20%, EG20% + GLY20% and EG40%, respectively. Experiment 3, (GV) buffalo oocytes were vitrified after exposure to (20% EG + 20% GLY or 20% EG + 20% DMSO) in straw, open pulled straw or solid surface vitrification system. The highest cleavage and blastocyst rates were obtained in SSV with 20% EG + 20% DMSO group (47.06% and 24.00%, respectively). These values were comparable to those recorded in the control group (61.22% and 46.90%, respectively). Experiment 4, no significant difference was noted between sucrose and trehalose warming media in cleavage and blastocyst rates (44.89% and 20.41% vs. 28.89% and 8.89% respectively). In conclusion, vitrification of buffalo (GV) oocytes using vitrification solutions composed of 20% EG + 20 DMSO without sucrose using SSV, then warming in a stepwise manner using either sucrose or trehalose is the best method for vitrification of (GV) buffalo oocytes |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Buffalo |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Oocyte |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Vitrification |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Aly Badawy Aly Badawy , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gamal Moustafa Darwish , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sayed Taha Ismail , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.14.M.Sc.2016.Am.S | 01010110069091000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.14.M.Sc.2016.Am.S | 01020110069091000 | 22.09.2023 | CD - Rom | 69091.CD |