In vitro Development of Camel (Camelus dromedarius) Embryos Applying Intracytoplasmic Sperm Injection / (Record no. 56759)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 03404cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031517.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160602s2015 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.07.03.M.Sc.2015.Ah.I |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ahmed Mohamed Kamel |
| 245 10 - TITLE STATEMENT | |
| Title | In vitro Development of Camel (Camelus dromedarius) Embryos Applying Intracytoplasmic Sperm Injection / |
| Statement of responsibility, etc. | Ahmed Mohamed Kamel ; Supervised Gamal Ashour Hassan , Ashraf Abdelhalim Elsayed , Khalid Ahmed Abdelsadek Elbahrawy |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تطور أجنة الإبل وحيدة السنام معمليا بإستخدام تقنية الحقن المجهرى |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Ahmed Mohamed Kamel , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 75 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Agriculture - Department of Animal Production |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The current study was conducted to evaluate the effects of maturation time (30 or 40 hours) and effect of LH addition (10 æl/ml) to in vitro maturation medium on maturation rate (%) of dromedary camel oocytes. Also, to investigate the effect of using two sources of spermatozoa; epididymal spermatozoa (G1), frozen semen (G2). In addition, to study the effect of two insemination methods: conventional IVF and intracytoplasmic sperm injection (ICSI) on the cleavage rate and development competence of in vitro produced embryos as a trial to improve the reproductive efficiency of the dromedary camel. Cumulus oocytes complexes (COCs) were recovered from ovaries by slicing technique. Based on morphological characteristics (number of cumulus cells enclosed the oocytes and the homogeneity of the cytoplasm), only grade A and B oocytes were selected to be cultured in TCM- 199 medium for maturation at 38.5 {u1D52}C, 5% CO and 95% humidity in air for 30 or 2 40 hours according to maturation protocol. The mature oocytes were subjected to two sources of spermatozoa. For G1, a number of 205 oocytes were inseminated with fresh epididymal spermatozoa (1x106 spermatozoa/ml). In G2, a number of 290 oocytes were inseminated with frozen thawed semen (3x106 spermatozoa/ml). Whereas in ICSI group, 28 oocytes were denuded and inseminated (individually injected) by ICSI technique. The results showed a significantly (P{u2264}0.05) higher maturation rate (83%) in oocytes subjected to 30 h maturation compared to 40 h group (64%). On the other hand, the results showed no significant effect for LH addition between the two groups. There was no significant (P> 0.05) difference in cleavage and blastocyst rates between the two groups of sperm sources being (21%, 8.27% and 20.7%, 8.33%) for G1, G2 respectively. For fertilization techniques there was no significant (P> 0.05) difference in cleavage rate and blastocyst rate, between conventional IVF (20.9%, 8.3%) and ICSI (23%, 5.5%) respectively. |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Dromedary |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Fertilization protocols |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | ICSI |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ashraf Abdelhalim Elsayed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gamal Ashour Hassan , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Khalid Ahmed Abdelsadek Elbahrawy , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Soheir |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.07.03.M.Sc.2015.Ah.I | 01010110069119000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.07.03.M.Sc.2015.Ah.I | 01020110069119000 | 22.09.2023 | CD - Rom | 69119.CD |