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Studies of cryopreservation of dog semen / (Record no. 57069)

MARC details
000 -LEADER
fixed length control field 03552cam a2200337 a 4500
003 - CONTROL NUMBER IDENTIFIER
control field EG-GiCUC
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250223031526.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 160703s2016 ua dh f m 000 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EG-GiCUC
Language of cataloging eng
Transcribing agency EG-GiCUC
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
049 ## - LOCAL HOLDINGS (OCLC)
Holding library Deposite
097 ## - Thesis Degree
Thesis Level Ph.D
099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC)
Classification number Cai01.10.14.Ph.D.2016.Al.S
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Ali Salama Hassan Soliman
245 10 - TITLE STATEMENT
Title Studies of cryopreservation of dog semen /
Statement of responsibility, etc. Ali Salama Hassan Soliman ; Supervised Mohamed Samy Sayed Abdou , Maha Soliman Ziada
246 15 - VARYING FORM OF TITLE
Title proper/short title دراسات علي حفظ السائل المنوى للكلاب بالتجميد
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Cairo :
Name of publisher, distributor, etc. Ali Salama Hassan Soliman ,
Date of publication, distribution, etc. 2016
300 ## - PHYSICAL DESCRIPTION
Extent 168 P. :
Other physical details charts , facsimiles ;
Dimensions 25cm
502 ## - DISSERTATION NOTE
Dissertation note Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Theriogenology
520 ## - SUMMARY, ETC.
Summary, etc. Different aspects of cryopreservation of dog semen have been investigated for the first time in Egypt. Studies were conducted on ejaculated semen (38 dogs) and epididymal sperm (20 dogs) during the period from July 2013 till October 2015. Ten experiments were done to determine the effect of non-canine commercial (BullXcell and INRA-96), Tris and milk based extenders, different cryoprotectants viz., glycerol, dimethyl sulfoxide (DMSO), dimethyl formamide, (DMF) ethylene glycol (EG) and propylene glycol (PG), egg yolk from different avian species (chicken, duck, goose and ostrich), replacing of egg yolk in semen extender by low density lipoprotein (LDL), centrifugation, packaging size, freezing rate and thawing rate on sperm cryosurvival, possibility of cryopreservation of epididymal sperm and extent of damage imposed on sperm by freezing and thawing. Results were evaluated by pre-freeze sperm motility, post-thaw sperm motility and viability, acrosomal integrity, hypo-osmotic swelling test and in vitro-fertilization. It was found that, Tris-based extenders were superior to milk-based ones and that BullXcell, could successfully be used for dog semen cryopreservation. In Tris extenders, fructose was superior to glucose. Moreover, addition of trehalose or lecithin to the extender improved the post-thaw semen quality. DMSO had a cryoprotective role comparable to glycerol; both were better than other cryoprotectants. Duck egg yolk improved freeze-thaw semen quality compared to egg yolk from chicken, ostrich or goose. LDL could successfully substitute egg yolk in dog semen extenders. Centrifugation and packaging of semen in 0.25ml or 0.50 ml straws had no significant effect on post-thaw quality. Freezing of semen in LN vapor, 5 cm above the surface of LN for 10 min was superior to freezing at a faster rate. Thawing of frozen semen at 70 OC for 6 sec gave better results than thawing at slower or faster rates. Epididymal sperm could successfully be frozen using the same protocol as that for ejaculated semen. The freezability of semen from individual dogs is important factor in the success of any cryopreservation program. Freeze-thaw associated ultrastructural changes in the head and mid-piece regions of spermatozoa were similar to those found in other animal species. Further studies are required to assess their effect on fertility
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE
Additional physical form available note Issued also as CD
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Cryopreservation
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Dog
653 #4 - INDEX TERM--UNCONTROLLED
Uncontrolled term Semen
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Maha Soliman Ziada ,
Relator term
700 0# - ADDED ENTRY--PERSONAL NAME
Personal name Mohamed Samy Sayed Abdou ,
Relator term
856 ## - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a>
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Enas
Reviser Cataloger
905 ## - LOCAL DATA ELEMENT E, LDE (RLIN)
Cataloger Nazla
Reviser Revisor
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Thesis
Holdings
Source of classification or shelving scheme Not for loan Home library Current library Date acquired Full call number Barcode Date last seen Koha item type Copy number
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة قاعة الرسائل الجامعية - الدور الاول 11.02.2024 Cai01.10.14.Ph.D.2016.Al.S 01010110069341000 22.09.2023 Thesis  
Dewey Decimal Classification   المكتبة المركزبة الجديدة - جامعة القاهرة مخـــزن الرســائل الجـــامعية - البدروم 11.02.2024 Cai01.10.14.Ph.D.2016.Al.S 01020110069341000 22.09.2023 CD - Rom 69341.CD