Oocyte competence and in vitro produced embryos in camels (camelus dromedarius) : (Record no. 57848)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 03410cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160925s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.14.M.Sc.2016.Ah.O |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ahmed Abdelfattah Mohammed Abdelfattah |
| 245 10 - TITLE STATEMENT | |
| Title | Oocyte competence and in vitro produced embryos in camels (camelus dromedarius) : |
| Remainder of title | With emphasis on gene expression / |
| Statement of responsibility, etc. | Ahmed Abdelfattah Mohammed Abdelfattah ; Supervised Nabil A. Hemeida , Mohamed A. I. Elsayed , Omaima M. T. Kandil |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | كفاءه البويضات والأجنه المنتجه معملياً فى الجمال : |
| Remainder of title | مع التركيز على التعبير الجيني |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Ahmed Abdelfattah Mohammed Abdelfattah , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 149 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Theriogenology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Six experiments were conducted on dromedary camels during the breeding (November to April) and non-breeding (May to October) seasons for two successive years (2013/2014 and 2014/2015) in order to investigate factors affecting oocyte in vitro developmental competence and to investigate gene expression of matured oocytes. Selected (excellent and good quality) oocytes were cultured in TCM-199 and CR1aa media at 38.5 {u00BA}C, 5% CO for 40 hrs, without or with EGF. Higher 2 (P<0.05) oocyte yield rate of excellent and good quality was observed during the breeding versus the non-breeding season. Camel oocytes cultured in TCM-199 showed better cytoplasmic maturation rate than CR1aa as expressed by increased percentage of GIII cumulus expansion. TCM-199 medium supplemented with EGF increased nuclear maturation rate versus its effect in CR1aa. Moreover, addition of EGF to TCM-199 and CR1aa improved cytoplasmic maturation rate. Cleavage rate of IVF oocytes increased in maturation medium TCM-199 + EGF when compared with TCM-199 without EGF. Natural parthinod camel embryos were higher during the breeding season in zygote (2nd pb), 2- cell, 4-cell and 8-cell stages and embryo like structure when compared with the non-breeding season. Significantly (p<0.05) higher CX43, IGF1, ATP5A1 were found in matured oocytes of TCM-199+EGF group than immature and TCM-199 groups. BCL2 (apoptosis gene) was significantly (P<0.05) higher in TCM-199 camel oocytes group than that observed in TCM-199+EGF immature oocytes group. In conclusion, breeding season was characterized by excellent and good oocyte yield.TCM- 199 and CR1aa media were suitable for in vitro maturation of camel oocytes. IVF of matured camel oocytes can be done using epididymal sperm. Addition of EGF to culture media improved developmental competence of camel oocytes and embryos due to its effect on gene expression of matured oocytes. Natural parthinogenesis phenomenon was higher during the breeding season: |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Dromedary camel |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | In vitro maturation |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Oocyte competence |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed Ahmed Ismail Elsayed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nabil Abdelmoneim Hemeida , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Omaima Mohamed Tawfek Kandil , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Soheir |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.14.M.Sc.2016.Ah.O | 01010110069706000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.14.M.Sc.2016.Ah.O | 01020110069706000 | 22.09.2023 | CD - Rom | 69706.CD |