Pathology and pathogenesis of metastasis in novel nude mouse model of feline mammary cancer / (Record no. 57884)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03922cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031551.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160929s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.05.Ph.D.2016.Ba.P |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Bardes Bahaa Abdelhakim Hassan |
| 245 10 - TITLE STATEMENT | |
| Title | Pathology and pathogenesis of metastasis in novel nude mouse model of feline mammary cancer / |
| Statement of responsibility, etc. | Bardes Bahaa Abdelhakim Hassan ; Supervised Sohair Mahmoud Sokkar , Iman Bakr Mohamed , Kawkab Abdelaziz Ahmed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | باثولوجيا التطور المرضى لانتشار سرطان الثدى القططى فى نماذج الفئران |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Bardes Bahaa Abdelhakim Hassan , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 131 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Clinical Pathology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Feline mammary carcinoma (FMC) is similar to human breast cancer (HBC) in the late age of onset, incidence, histopathologic features, biologic behavior, and pattern of metastasis. There is a lack of knowledge about the existence and prognostic value of lymphangiogenesis in FMC. Therefore, FMC has been proposed as a relevant model for aggressive HBC. The goals of this study were to 1) develop a nude mouse model of FMC growth and metastasis; 2) measure the expression of genes responsible for lymphangiogenesis, angiogenesis, tumor progression and lymph node metastasis using FMC tissues, mouse xenografts and cell lines; and 3) design an immunohistochemical protocol to differentiate between blood and lymphatic vessels in FMC and to compare blood and lymphatic vessel invasion detected by hematoxylin and eosin (H&E) versus that detected by immunohistochemistry (IHC). Two primary FMC tissues were injected subcutaneously and six FMC cell lines were injected into 3 sites (subcutaneous, intratibial and intracardiac) in nude mice. Tumors and metastases were monitored using bioluminescent imaging and characterized by gross necropsy, radiology and histopathology. Molecular characterization of invasion and metastasis genes in FMC was conducted using qRT-PCR in six primary FMC tissues, two subcutaneous FMC xenografts and six FMC cell lines. A survey study was done on 42 specimens from cats diagnosed with mammary cancer were stained with H&E and classified based on histopathological examination. Eighteen specimens out of 42, characterized by vascular and/or lymphatic invasion, were selected and evaluated by IHC using antibody against prospero-related homeobox domain 1 (PROX1) as lymphatic endothelial marker. The histologic appearance of the subcutaneous xenografts resembled the primary tumors. No metastasis was evident following subcutaneous injection of both tumor tissues and cell lines while lung, brain, liver, kidney and bone metastases were confirmed following intratibial and intracardiac injection of FMC cell lines. Fifteen genes were differentially expressed in the FMC tissue and cell lines and the highly expressed genes in all the samples were PDGFA, PDGFB, PDGFC, FGF2, EGFR, ERBB2, ERBB3, VEGFD, VEGFR3 and MYOF. Finally, PROX1 immunostaining was present in the nucleus of the peritratumoral lymphatic endothelial cells. This investigation demonstrated the usefulness of nude mouse models of experimental FMC and identified molecular targets of FMC progression and metastasis |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Lung |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Mammary cancer |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Metastasis |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Iman Bakr Mohamed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kawkab Abdelaziz Ahmed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sohair Mahmoud Sokkar , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Soheir |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.05.Ph.D.2016.Ba.P | 01010110069711000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.05.Ph.D.2016.Ba.P | 01020110069711000 | 22.09.2023 | CD - Rom | 69711.CD |