MARC details
| 000 -LEADER |
|---|
| fixed length control field |
03183cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
161013s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
Ph.D |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.19.03.Ph.D.2016.Ma.R |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Marwa Mohamed Fathi Ahmed Elswify |
| 245 14 - TITLE STATEMENT |
|---|
| Title |
The role of PCR assay compared to conventional laboratory methods in the diagnosis of invasive fungal infections / |
| Statement of responsibility, etc. |
Marwa Mohamed Fathi Ahmed Elswify ; Supervised Hadir Ahmed Elmahalawy , Mohamed Tarek Mansour , Maggie Louis Naguib |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
دور التفاعل البلمرى المتسلسل بالمقارنة مع الإختبارات المعملية التقليدية فى تشخيص عدوى الفطريات العميقة |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Marwa Mohamed Fathi Ahmed Elswify , |
| Date of publication, distribution, etc. |
2016 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
181 P. : |
| Other physical details |
charts , facsimiles ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (Ph.D.) - Cairo University - National Cancer Institute - Department of Clinical Pathology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
The present study was carried out at the National Cancer Institute, Cairo University, on bronchoalveolar lavage (BAL) samples received from sixty pediatric Cancer patients with suspected lower respiratory tract IFI in the period between January 2013 and May 2015. Patients included in the study were subjected to clinical examination, radiological and laboratory investigations. BAL samples received at microbiology laboratory were divided into 3 portions on which the following tests were performed: Direct microscopic examination, culture on blood agar, MacConkey agar and specific fungal media with identification of detected growth to the species level and antimicrobial susceptibility testing for bacterial isolates, Galactomannan antigen detection by Platelia{u2122} Aspergillus Ag Kit and pan fungal PCR Assay. Fungal DNA extraction was performed using QIAamp DNA Mini Kit, lyticase enzyme was utilized for enzymatic lysis of fungal cell wall. PCR amplification was performed utilizing the universal fungal primers ITS1 and ITS4. Detection of PCR amplified products was done by agarose gel electrophoresis. Patients were classified according to a modified approach of the revised definitions of IFI from the EORTC/MSG into: proven, probable, possible and unlikely IFI.Results: Various risk factors were associated with the development of IFI including hematological malignancies (P-value = 0.014), active disease state, Profound neutropenia <500/mm³ for more than 10 days (p-value <0.001), prolonged use of corticosteroids for > 3 weeks (p-value=0.035). Aspergillus growth was the predominant causative agent of lower respiratory tract IFI in our study. The most common isolated Aspergillus species were A. fumigatus and A. flavus, followed by A. nidulans, A. terreus and A. glaucus |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Galactomannan |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
IFI |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
PCR |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Hadir Ahmed Elmahalawy , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Maggie Louis Naguib , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mohamed Tarek Mansour , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Enas |
| Reviser |
Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
