Optimization of gene delivery using laser and nanomaterials : (Record no. 58351)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02940cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031605.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 161029s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.24.05.M.Sc.2016.Ma.O |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Marina Atef George Adeeb |
| 245 10 - TITLE STATEMENT | |
| Title | Optimization of gene delivery using laser and nanomaterials : |
| Remainder of title | In vitro study / |
| Statement of responsibility, etc. | Marina Atef George Adeeb , Tareq Youssef , Salwa Farouk Sabet ,Taher Ahmed Salaheldin, |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسة معملية لتحسين النقل الجينى باستخدام الليزر والمواد النانوية |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Marina Atef George Adeeb , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 89 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Sciences - Department of Laser Applications in Environmental Metrology, Photochemistry and Agriculture |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The present work aimed to prepare stable gelatin nanoparticles conjugated with the nonstructural protein 2 (NS2) gene of hepatitis C virus (HCV) genotype 4a as an extra efficient method of vaccine delivery where nucleic acids can be loaded onto GNPs through electrostatic attraction. We synthesized GNPs by two step desolvation method and resulted were found to be of spherical shape, of size around 150±2nm and with zeta potential of +17.6 mv. We amplified NS2 gene form HCV genome (strain ED43), then cloned and expressed it in E.coli M15 using pQE-30 vector. Antigenicity of the recombinant NS2 protein was confirmed by Western blotting to verify the efficiency of NS2 as a possible vaccine before conjugation with GNPs. We successfully conjugated NS2 gene and GNPs and that was confirmed by confocal laser scanning microscope. We then investigated effects of conjugated GNPs/NS2 complex on transformation in E.coli DH5Ü. According to many trials, we found that the conjugated GNPs/NS2 with ratio (2:1) respectively was transformed into E.coli DH5Ü more efficiently as gene delivery system than NS2 transformation alone or other prepared ratios (1:2, 1:1). We confirmed the complex delivery using confocal laser scanning microscope excited by three lines of laser 633nm, 405nm and 514nm.Moreover, we concluded that GNPs didn{u201F}t affect the properties of NS2 gene verified using gel electrophoresis. This declared that we could succefully deliver NS2 gene using GNPs as first step for Vaccine development against HCV |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Gene delivery |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | GNPs |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Nanoparticles |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Salwa Farouk Sabet , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Taher Ahmed Salah Eldin , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Tareq Youssef , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Soheir |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.24.05.M.Sc.2016.Ma.O | 01010110069881000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.24.05.M.Sc.2016.Ma.O | 01020110069881000 | 22.09.2023 | CD - Rom | 69881.CD |