MARC details
| 000 -LEADER |
|---|
| fixed length control field |
02501cam a2200313 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
161112s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
EG-GiCUC |
| Language of cataloging |
eng |
| Transcribing agency |
EG-GiCUC |
| 041 0# - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
Deposite |
| 097 ## - Thesis Degree |
|---|
| Thesis Level |
M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) |
|---|
| Classification number |
Cai01.12.21.M.Sc.2016.Ma.C |
| 100 0# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Maye Mamdouh Abdulatif Khalifa |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Cloning of a molecular vector encodes for the Hemagglutinin gene of the Egyptian strain of avian influenza A virus (H5N1) / |
| Statement of responsibility, etc. |
Maye Mamdouh Abdulatif Khalifa ; Supervised Mohamed Akmal Elghor , Mohamed Mustafa Mustafa Ibrahim |
| 246 15 - VARYING FORM OF TITLE |
|---|
| Title proper/short title |
(H5N1) كلونة ناقل جزيئي يشفر لجين الهيماجلوتينين من سلالة مصرية لفيروس انفلونزا الطيور |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
Cairo : |
| Name of publisher, distributor, etc. |
Maye Mamdouh Abdulatif Khalifa , |
| Date of publication, distribution, etc. |
2016 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
123 P. : |
| Other physical details |
charts , facsimiles ; |
| Dimensions |
25cm |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
The spread of highly pathogenic avian H5N1 influenza viruses since 1997 and their virulence for poultry and humans has raised concerns about their potential to cause an influenza pandemic. Early detection of newly circulating viral strains as well as vaccination offers the most viable means to combat a pandemic threat. However, it will be a challenge to produce, distribute and implement a new vaccine if a pandemic spreads rapidly. The current study aimed at isolation of the full length hemagglutinin gene via Polymerase Chain Reaction (PCR), cloning the isolated gene and finally sequencing the amplified fragment in order to identify the restriction fragment considered for further expression cloning experiments. In order to achieve these objectives, a real time PCR protocol specific to the Egyptian H5N1 strains has been developed and tested on both in silico and in vitro levels. Moreover, bioinformatics analysis of generated DNA sequence data showed that the target H5N1 isolate is closely related to the other Egyptian H5N1 strains specially, strain (Egypt-MOH-NCR-7305). Finally, restriction recognition sequences inside the entire full length H5 gene have been identified |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE |
|---|
| Additional physical form available note |
Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
H5N1, Egyptian |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Hemagglutinin |
| 653 #4 - INDEX TERM--UNCONTROLLED |
|---|
| Uncontrolled term |
Real time PCR |
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mohamed Akmal Elghor , |
| Relator term |
|
| 700 0# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mohamed Mustafa Mohamed Ibrahim , |
| Relator term |
|
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Nazla |
| Reviser |
Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) |
|---|
| Cataloger |
Soheir |
| Reviser |
Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Thesis |
