Molecular biological studies on Escherichia coli isolated from diarrheic and apparently healthy calves / (Record no. 58798)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03188cam a2200313 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 161127s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.09.M.Sc.2016.No.M |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Noha Mohamed Bakry Nady |
| 245 10 - TITLE STATEMENT | |
| Title | Molecular biological studies on Escherichia coli isolated from diarrheic and apparently healthy calves / |
| Statement of responsibility, etc. | Noha Mohamed Bakry Nady ; Supervised Walid Sayed Ahmed Abdelkader Awad , Amr Abdelaziz Abdelkader Elsayed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات بيوجزيئية عن الايشيريشيا كولاى العزولة من عجول مصابة بالاسهال و عجول سليمة ظاهريا |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Noha Mohamed Bakry Nady , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 116 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Diseases |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Neonatal calf diarrhea is one of the most important problems in young calves causing great economic losses. In the current study, a total number of 150 fecal samples from diarrheic and apparently healthy in-contact cattle and buffalo calves under 3 months old were examined bacteriologically for isolation of E. coli. All isolates were confirmed by PCR using 16SrRNA gene. Multiplex PCR was applied for detection of virulence genes and for studying the association of these genes with different E. coli pathotypes. One hundred and six of 150 isolates were positive for at least one virulence gene. The rate of f , st, lt, eae, stx , stx , hylA and astA genes were 17.3%, 27.3%, 6.7%, 10%, 37.3%, 5 1 2 17.7%, 9.3% and 20.7%, respectively. Distribution of E. coli pathotypes revealed presence of ETEC, EPEC, STEC, EAEC, ET.EP. and ET.ST. at a rate of 12.7%, 2.7%, 30.7%, 7.3%, 2.7% and 14.7%, respectively. The rate of pathogenic E. coli infection in calves was higher in winter season and in the first weeks of life. Molecular serogrouping of E. coli isolates was carried using 10 O serogroups and revealed presence of O91 serogroup in the highest rate (62.7%), with higher rate of O26, O91, O121 and O45 in diarrheic calves. Comparing between molecular serogrouping and agglutination test revealed the ability of multiplex PCR to detect all isolates which were positive by agglutination and also 8 negative isolates. Five intestinal samples from four dead calves grossly showing congestion and distention of intestinal loops were bacteriologicaly positive for E. coli with higher rate of eae gene. Histopathological examination revealed heamorrhagic enteritis, shortening and fusion of intestinal villi, desquamation of lining epithelium of intestinal mucosa with congestion of blood capillaries and intense leucocytic infiltration |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Calves |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Diarrhea |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Multiplex PCR |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Amr Abdelaziz Abdelkader Elsayed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Walid Sayed Ahmed Abdelkader Awad , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Soheir |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.09.M.Sc.2016.No.M | 01010110070383000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.09.M.Sc.2016.No.M | 01020110070383000 | 22.09.2023 | CD - Rom | 70383.CD |