A novel transformation system, based on laser micro puncturing beam and gene coated gold particles in wheat / (Record no. 59663)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02494cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031645.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 170204s2016 ua h f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.24.05.M.Sc.2016.Ma.N |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Mahmoud Said Hamed Ali Amer |
| 245 12 - TITLE STATEMENT | |
| Title | A novel transformation system, based on laser micro puncturing beam and gene coated gold particles in wheat / |
| Statement of responsibility, etc. | Mahmoud Said Hamed Ali Amer ; Supervised Mona A. Aziz , Reda Abdelhalim |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | منظومة مبتكرة للنقل الجينى مبنية على شعاع الليزر الميكرونى و جسيمات الذهب المطعمة بالجين فى القمح |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Mahmoud Said Hamed Ali Amer , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 132 P. : |
| Other physical details | facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Applications in Environmental Photochemistry and Agriculture |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | We report new transfection methods for intact plant cells that uses laser beam to introduce foreign gene-coated gold nanoparticles into wheat mature embryos cells (Triticum aestivum) through the cell wall and cell membrane. This homemade setup secures the transformation of as many as 30 mature embryo derived calli in less than one hour using second harmonic of Nd:YAG laser at 532nm(150mW) with two dimensional translation stages, a suitable computer program and a proper optical system. Seven days old calli were irradiated by a focused laser microbeam to puncture momentarily made self healing holes ({uF07E}0.5 æm) in the cell wall and membrane to allow uptake of foreign genes coated gold nanoparticles. The plant expression vector PBI-121 harboring the green fluorescent protein gene (gfp) under the control of 35-S promoter and nos terminator and the neomycin phosphotransferase-II gene (npt-II) as a selectable marker for the herbicide kanamycin sulfate resistance. Treated wheat tissue samples were then cultured for several weeks on the selection medium |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Laser mediated gene transfer |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Mature embryos |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Wheat |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mona Abdelaziz Mohamed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Reda Elwany Abdelhalim , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Samia |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.24.05.M.Sc.2016.Ma.N | 01010110070998000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.24.05.M.Sc.2016.Ma.N | 01020110070998000 | 22.09.2023 | CD - Rom | 70998.CD |