Genes regulate oocyte competence and embryo development in the buffalo / (Record no. 60019)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03557cam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 170227s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.10.14.M.Sc.2016.Es.G |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Esraa Aly Sayed Ismail |
| 245 10 - TITLE STATEMENT | |
| Title | Genes regulate oocyte competence and embryo development in the buffalo / |
| Statement of responsibility, etc. | Esraa Aly Sayed Ismail ; Supervised Mohamed A. I. Elsayed , Nabil A. Hemeida , Omaima M. Kandil |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | الجينات التى تنظم نمو البويضات والأجنة فى الجاموس |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Esraa Aly Sayed Ismail , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 157 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Theriogenology |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This work was carried out for two successive years (2014 and 2015) in order to investigate gene expression of in vitro matured oocytes and blastocysts in buffaloes as well as to study factors affecting the buffalo oocyte and embryo developmental competence. Selected (excellent and good quality) oocytes were cultured in TCM- 199 medium at 38.5 {u00BA}C, 5% CO2 for 24 hrs. Supplementation of maturation medium TCM with IGF-1 increased significantly (P<0.05) the oocyte maturation, cleavage and transferable embryos rates (morula and blastocyst). ZFP64, BMP15 and ATP5A1, gene expression was increased (p<0.05) in buffalo oocytes matured in TCM- 199+IGF. Co-culture of fertilized oocytes using BOEC during in vitro culture of fertilized oocytes increased (P<0.05) the cleavage rate, transferable embryos rate (morula and blastocyst) and upgraded (CX43, CDX2, Oct4, INF-tu) gene expression in blastocysts of buffaloes. BCL2 (Anti-apoptosis gene) was higher (P<0.05) in blastocysts cultured on the SOF group than that observed in SOF with BOEC group. Higher (P<0.05) oocyte yield rate of excellent and good quality was observed during winter versus summer season. Acute heat shock for one hour during in vitro maturation, significantly (P<0.05) decreased the maturation rate and the cleavage rate in buffaloes. IGF-1 supplement in maturation media of acute heat shocked oocyte led to a significant increase (P<0.05) in the maturation rate, the cleavage rate and transferable embryos than matured without IGF. Co-culture (BOEC) in culture medium of acute heat shocked oocyte significantly increased the cleavage rate and blastocyst rate than cultured in SOF. In conclusion, supplementation of maturation media with IGF-1 improved developmental competence of buffalo oocytes and embryos due to its effect on gene expression. Co-culture (BOEC) during in vitro early embryo development significantly increased the transferable embryo rate (blastocyst) in buffaloes due to subsequent upgrade genes regulating early embryonic development (CX43, Oct4, CDX2, INF-tu and BCL2). Acute heat shock significantly decreased oocyte and embryo developmental competence in buffaloes. Both of IGF-1 in maturation medium and co-culture (BOEC) secure the acute heat shock effect on oocyte and embryo developmental competence in buffaloes |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Buffalo |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | In vitro maturation |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Oocyte competence |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohamed A. I. Elsayed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nabil A. Hemeida , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Omaima M. Kandil , |
| Relator term | |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shaima |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.10.14.M.Sc.2016.Es.G | 01010110071179000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.10.14.M.Sc.2016.Es.G | 01020110071179000 | 22.09.2023 | CD - Rom | 71179.CD |