Evaluation of the efficacy of human antibodies against hepatitis C virus antigens / (Record no. 60384)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03352cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031706.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 170325s2016 ua dh f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.12.02.M.Sc.2016.Em.E |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Eman Ahmed Salem Ahmed Reda |
| 245 10 - TITLE STATEMENT | |
| Title | Evaluation of the efficacy of human antibodies against hepatitis C virus antigens / |
| Statement of responsibility, etc. | Eman Ahmed Salem Ahmed Reda ; Supervised Sanaa Osman , Amr Saad , Ashraf Abdou Tabll |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تقييم فعالية الأجسام المضادة البشرية ضد أنتجينات فيروس الكبد الوبائى سى |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Eman Ahmed Salem Ahmed Reda , |
| Date of publication, distribution, etc. | 2016 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 125 P. : |
| Other physical details | charts , facsimiles ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biochemistry |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Hepatitis C virus (HCV) is a major health problem worldwide particularly in Egypt. The humoral immune response has an important function in the control of HCV infection. To study the role of neutralizing antibodies in Hepatitis C Virus (HCV) clearance in infected individuals. This study was carried out on apparently healthy blood donors (n=200). Detectable HCV antibodies were assessed by commercial ELISA. Specific human immunoglobulin targeting peptides derived from HCV E1 /E2 regions in blood samples were measured by in house optimized ELISA. Human IgG purification was carried out from both samples positive and negative for HCV RNA in order to evaluate its neutralizing activity invitro using Huh 7 cells. The studied cohort included 96/200 subjects who were positive for HCV antibodies, among which: 56/96 (58%) samples were positive for HCV RNA (group 1) and 40/96 (42%) samples had undetectable HCV RNA (group 2). ELISA results showed that Human HCV Immunoglobulin (HHI) targeting HCV E1 synthetic peptide (a.a 315- 323) was detectable in 63/96 (66%) and HHI targeting HCV E2 (a.a 412- 419) were positive in 14/96 (15%) while19/96 (19%) were positive for HCV E2 (a.a 517-531). (HHI) higher than the cutoff level against peptide HCV E1 (a.a 315-323) were detected in 22/63 (35%) in blood donors group 2 and positive in 41/63 (65%) in group 1. HHI against peptide HCV E2 (a.a 412-419) were positive in 7 (50%) in blood donors group 2 and also positive in 7 (50%) of group 1. In addition HHI targeting HCV E2 (a.a517-531) were positive in 11 (60%) in group 2 compared with 8 cases (40%) in group 1. Purified human antibodies from cases positive for HCV antibodies and negative for HCV RNA showed invitro neutralization at concentrations 30 and 10 æg/ml while the same concentration of purified human IgG from cases positive for HCV RNA showed no viral neutralization. The tested epitope (s) derived from HCV envelope E1 and E2, are important for viral clearance and hence can be used for HCV vaccine development |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Antigens |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Hepatitis C virus |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Human antibodies |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Amr Saad , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ashraf Abdou Tabll , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sanaa Osman , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Shaima |
| Reviser | Cataloger |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.12.02.M.Sc.2016.Em.E | 01010110071474000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.12.02.M.Sc.2016.Em.E | 01020110071474000 | 22.09.2023 | CD - Rom | 71474.CD |