Role of LASER irradiation in genetic transformation of yeast / (Record no. 61221)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02727cam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031733.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 170614s2015 ua do f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.24.05.M.Sc.2015.Es.R |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Esraa Ragab Ahmed Elsharqawy |
| 245 10 - TITLE STATEMENT | |
| Title | Role of LASER irradiation in genetic transformation of yeast / |
| Statement of responsibility, etc. | Esraa Ragab Ahmed Elsharqawy ; Supervised Mona Abdelaziz Mohamed , Naglaa Abdelmonem Ahmed |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دور التشعيع بالليزر فى التحول الوراثى فى الخميرة |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Esraa Ragab Ahmed Elsharqawy , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 132 P. : |
| Other physical details | charts , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Application in Environmental Metrology Photochemistry and Agriculture |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In the current study, the application of near-infrared diode laser irradiation as a new method for gene delivery in yeast has been studied and compared with chemical (LiAc) as a traditional method. The two treatments have been carried out on Saccharomyces cerevisiae (strain,NCYC1611a) with pBS4 plasmid DNA carrying hygromycin B resistance (hphr) andcyano-fluorescent protein (CFP) genes. This study aimed to establish a novel transformation method using 808nm near IR diode laser irradiation to facilitate introducing exogenous reporter genes in yeast. Results showed slight differences between the two method.NIR diode laser irradiation methods proved the best vs. LiAc chemical method because it showed less ultra- damaging and less cytotoxic effects compared to the control normal yeast.Histological and molecular analysis proved the presence, and expression of both hphr and CFP genes in the two methods. Molecular laser Raman Spectroscopy (MLRS) confirmed the presence of the pBS4 plasmid, containing both trans-genes in transformed yeast by the two methods. Also confocal laser scanning microscopy (CLSM) proved the expression of CFP gene. Ultra- damages was detected using transmission electron microscopy (TEM), Raman spectroscopy was usedalso to detect the damages on DNAmolecular scale for both methods vs. normal yeast cells |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Near-IR diode laser irradiation |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | pBS4with CFP&hphrtrans-genesLithium- acetate method |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Yeast |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mona Abdelaziz Mohamed , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Naglaa Abdelmonem Ahmed , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Enas |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.24.05.M.Sc.2015.Es.R | 01010110071848000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.24.05.M.Sc.2015.Es.R | 01020110071848000 | 22.09.2023 | CD - Rom | 71848.CD |