Diagnosis of honeybee diseases using PCR and laser spectroscopic techniques / (Record no. 61224)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02963cam a2200349 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | EG-GiCUC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250223031733.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 170614s2015 ua do f m 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-GiCUC |
| Language of cataloging | eng |
| Transcribing agency | EG-GiCUC |
| 041 0# - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | Deposite |
| 097 ## - Thesis Degree | |
| Thesis Level | M.Sc |
| 099 ## - LOCAL FREE-TEXT CALL NUMBER (OCLC) | |
| Classification number | Cai01.24.05.M.Sc.2015.Am.D |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Amal Mohamed Ibrahim Osman |
| 245 10 - TITLE STATEMENT | |
| Title | Diagnosis of honeybee diseases using PCR and laser spectroscopic techniques / |
| Statement of responsibility, etc. | Amal Mohamed Ibrahim Osman ; Supervised Mona Abdelaziz Mohamed , Emtithal Mohammed Abdelsamiea , Hamdy Taher Mohamed Abouelenain |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | و استطياف الليزر PCRتشخيص امراض نحل العسل باستخدام تقنيات ال |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Cairo : |
| Name of publisher, distributor, etc. | Amal Mohamed Ibrahim Osman , |
| Date of publication, distribution, etc. | 2015 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 152 P. : |
| Other physical details | charts , photographs ; |
| Dimensions | 25cm |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Thesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Application in Environmental Metrology Photochemistry and Agriculture |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Paenibacillus larvae subsp. larvae, the causative pathogenic bacterium of virulent brood disease (American foulbrood, AFB). DNA extracted from P. l. larvae strain was subjected to PCR using specific primers of a partial sequence of 16S rRNA gene that closely related to the bacterium. Assayed bacterial strain produced a defined band of 700 bp in size. This test was done based on DNA {u2013} extraction of bacterial colonies and different larval stages(1-5 days, of age) from Carniolan (Apis mellifera carnica) honeybee and Italian (Apis mellifera ligustica), The results of PCR analysis showed that the (day1) of all honeybee races didn't amplify the pathogen fragment (700bp) and all larval stages from (day2- day5) generated PCR fragment of 700bp. Also, this defined fragment was detected in larvae, pupae, adult worker and virgin queen resulted from the grafting Italian honeybee. On the other hand, the physicochemical structure of genomic DNA of pathogenic bacterium P. l. larvae and the two honeybee races before (-ve control) &after infection with the pathogen were identified and characterized qualitatively and quantitatively using molecular laser Raman (MLRS) and infra-red (FTIR) spectroscopies for the first time. The results of spectroscopic analysis clearly revealed that the pathogen was more abundant in Italian race from the early infection stage (day1) to (day5) of age. than Carniolan which showed more resistant to pathogen infection of the (day2) |
| 530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE | |
| Additional physical form available note | Issued also as CD |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | Carniolan and Italian honeybee races |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | P. l. larvae |
| 653 #4 - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | PCR |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Emtithal Mohammed Abdesamiea , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hamdy Taher Mohamed Abouelenain , |
| Relator term | |
| 700 0# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mona Abdelaziz Mohamed , |
| Relator term | |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://172.23.153.220/th.pdf">http://172.23.153.220/th.pdf</a> |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Enas |
| Reviser | Cataloger |
| 905 ## - LOCAL DATA ELEMENT E, LDE (RLIN) | |
| Cataloger | Nazla |
| Reviser | Revisor |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Thesis |
| Source of classification or shelving scheme | Not for loan | Home library | Current library | Date acquired | Full call number | Barcode | Date last seen | Koha item type | Copy number |
|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | قاعة الرسائل الجامعية - الدور الاول | 11.02.2024 | Cai01.24.05.M.Sc.2015.Am.D | 01010110071849000 | 22.09.2023 | Thesis | ||
| Dewey Decimal Classification | المكتبة المركزبة الجديدة - جامعة القاهرة | مخـــزن الرســائل الجـــامعية - البدروم | 11.02.2024 | Cai01.24.05.M.Sc.2015.Am.D | 01020110071849000 | 22.09.2023 | CD - Rom | 71849.CD |